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Fig. S1. Enzymatic analysis of the physical phage genome ends. Agarose gelelectrophoresis (1%) of SalI-digested 14-1 DNA after treatment with Bal31 for the indicated time intervals. Lanes: M, DNA marker (λ/PstI); a, untreated control DNA digested with SalI; b, 14-1 with Bal31 treatment for 10 min; c, 14-1 with Bal31 treatment for 20 min; d, 14-1 with Bal31 treatment for 40 min. Restriction fragments disappearing over time are indicated by arrows.

Fig. S2. Comparison of the nucleotide composition in the genomes of PB1-like phages using the Mauve algorithm. From top to bottom, phages 14-1, LBL3, LMA2, PB1, SN and F8 are aligned side by side. The height of the red graph level indicates the mutual conservation of the DNA sequence. Accordingly, drops to zero indicate specific insertions and/or deletions in the phage genomes. The vertical red line connects the middle base of each genome; the black-lined double boxes indicate the central open reading frames. Underneath the name of the phage are rulers in kb, and location of the predicted open reading frames.

Fig. S3. The construction of shotgun sequencing (SS) libraries resulted in clones of phages LMA2 and LBL3 which displayed a varying amount of G's in a homopolymer stretch. Primer walking (PW) on purified phage DNA produced mixed signals downstream this G-stretch, indicative for this mixed population. The same phenomenon was noticed in phage PB1.

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