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Fig. S1. Picture of the DGGE gels.

Fig. S2. Comparison of DGGE fingerprints of samples run on different gels. Highlighted in blue are the fingerprints from gels on which randomly chosen samples were loaded. The three other fingerprints are the three replicates corresponding to this sample (cut from Fig. S1; sometime including a marker lane).

Fig. S3. Graphical representation of the PCNM variables. The position of each data point is determined by the Universal Tansverse Mercator coordinates and the diameter of the bubble is proportional to the absolute value of the PCNM (see Table S1). Empty bubbles represent negative values and solid bubbles represent positive values (i.e. a large solid bubble is a large positive PCNM value, and a large empty bubble is a large negative PCNM value). The regions are indicated for the first PCNM and are the same for all PCNM.

Table S1. UTM coordinates of each of the 30 fields sampled and PCNM variables used in spatial analyses. CH, Chiltern Hills; SW, South Wessex Downs; SD, South Downs.

Table S2. Time since last cultivation and restoration strategy for each of the 30 fields sampled. CH, Chiltern Hills; SD, South Downs; SW, South Wessex Downs.

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