Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene
Version of Record online: 5 OCT 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 2, pages 304–314, February 2010
How to Cite
Louis, P., Young, P., Holtrop, G. and Flint, H. J. (2010), Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene. Environmental Microbiology, 12: 304–314. doi: 10.1111/j.1462-2920.2009.02066.x
- Issue online: 26 JAN 2010
- Version of Record online: 5 OCT 2009
- Received 23 June, 2009; accepted 11 August, 2009.
Fig. S1. Phylogenetic tree of reference organisms used in this study based on 16S rRNA gene sequences corresponding to positions 44–1491 of E. coli, which was used as the outgroup. Accession numbers for sequences are given in brackets. Bootstrap values are shown at branch points. The scale bar represents genetic distance (2 substitutions per 100 nucleotides).
Fig. S2. Comparison of thermal denaturation analysis of butyryl-CoA:acetate CoA-transferase gene amplicons with 16S rRNA gene-based and clone library-based results.
A. Regression of the observed melting temperature on the % G+C content of butyryl-CoA:acetate CoA-transferase gene amplicons from butyrate-producing isolates. Species names for strain designations are given in Fig. 1.
B. Regression of the melt curve areas between 82.5 and 85°C for E. hallii and SS2/1 (solid diamonds, R2 = 0.89), 85.5 and 89°C for Roseburia/E. rectale (open triangles, R2 = 0.62) and 89.5 and 92.5°C for F. prausnitzii (open squares, R2 = 0.61) on 16S rRNA gene-based real-time PCR results as expressed in Fig. 4C and D.
C. Regression of the melt curve areas as defined in B, on the butyryl-CoA:acetate CoA-transferase clone library data (solid diamonds, E. hallii and SS2/1, OTUs 13–15, 17–18, R2 = 0.66; open triangles, Roseburia/E. rectale, OTUs 1, 2, 4, 6–10, R2 = 0.42; open squares, F. prausnitzii, OTUs 26–29, R2 = 0.38). P < 0.001 for all regressions shown.
Table S1. Numbers of butyryl-CoA:acetate CoA-transferase clone sequences found per sample.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.