Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing
Article first published online: 16 OCT 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 2, pages 401–411, February 2010
How to Cite
Herrmann, S., Kleinsteuber, S., Chatzinotas, A., Kuppardt, S., Lueders, T., Richnow, H.-H. and Vogt, C. (2010), Functional characterization of an anaerobic benzene-degrading enrichment culture by DNA stable isotope probing. Environmental Microbiology, 12: 401–411. doi: 10.1111/j.1462-2920.2009.02077.x
- Issue published online: 26 JAN 2010
- Article first published online: 16 OCT 2009
- Received 20 November, 2008; accepted 31 August, 2009.
Vol. 13, Issue 2, 550, Article first published online: 1 FEB 2011
Fig. S1. A. Production of 13C-enriched carbon dioxide (mM) during the degradation experiment.
B. Isotope signatures of 13C-enriched carbon dioxide (closed) and 13C-enriched methane (open symbols) in individual microcosms amended with 13C-benzene during the degradation experiment. Diamonds: microcosm, 96% benzene degraded (initial benzene concentration: 0.74 mM); circles: microcosm, 72% benzene degraded (initial benzene concentration: 0.61 mM); squares: microcosm, 33% benzene degraded (initial benzene concentration: 0.58 mM).
Fig. S2. Phylogenetic relationship of the Sulfurovum-related clones (bold-faced) retrieved from the benzene-degrading consortium to representatives of the Epsilonproteobacteria (tree taken from Kleinsteuber et al., 2008, supporting information)
Table S1. Bacterial diversity within the microcosms after nearly complete benzene degradation (98% and 96% degradation of unlabelled and labelled benzene respectively): Lengths of T-RFs obtained after BstUI or RsaI digestion (bp), range of relative abundances within all 12C-benzene or 13C-benzene density gradient fractions, and phylogenetic assignment of the T-RF to cloned 16S rRNA genes reported by Kleinsteuber and colleagues (2008) or sequenced in this study (Table 2). The accession numbers of the according 16S rRNA gene sequences are given in parentheses.
Table S2. Sequencing results of representative 16S rRNA gene clones and according T-RF.
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