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Fig. S1. COG category distribution of deduced ANME-1 proteins and of the protein datasets of selected methanogenic archaea.

Fig. S2. Possible explanations for the lack of Mer.

Fig. S3. Overview of contig regions carrying candidate genes for Hdr-homologues.

Fig. S4. Overview of amino acid biosynthesis pathways in ANME-1.

Table S1. Overview of all fosmids included in the draft genome sequence of ANME-1.

Table S2. Overview of the assembled ANME-1 contigs.

Table S3. Overview of ANME-1 fosmids not included in the assembly.

Table S4. Overview of repetitive elements.

Table S5. List of methanogenic archaea used as a reference dataset for genome comparison.

Table S6. List of PFAM models present in the deduced protein dataset of ANME-1, but not in any protein dataset of 20 completely sequenced methanogenic archaea.

Table S7. List of Orfs related to proteins of the methanogenesis pathway.

Table S8. List of Orfs putatively involved in electron transfer and energy gain.

Table S9. List of Orfs putatively involved in the central carbon metabolism.

Table S10. List of Orfs putatively involved in amino acid biosynthesis.

Table S11. List of Orfs putatively involved in sulfur metabolism.

Table S12. List of Orfs putatively involved in nitrogen metabolism.

Table S13. List of Orfs putatively involved in stress response.

Table S14. Messenger RNA expression.

Table S15. List of 169 Pfam models occurring only once per genome in 20 methanogenic archaea (E-value cut-off 1E-4).

Table S16. A mapping of the list of universally distributed COGs (Ciccarelli et al., 2006) to Pfam models.

Table S17. Presence of Pfam models, deduced from the list of universally distributed COGs (Ciccarelli et al., 2006), in ANME-1 and in 20 methanogenic archeae (E-value cut-off: 1E-4).

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