Present addresses: IMEP, Université de Provence, Marseille, France; ‡LMBM, Université Mohammed V, Rabat, Maroc.
Diversity analyses of Aeschynomene symbionts in Tropical Africa and Central America reveal that nod-independent stem nodulation is not restricted to photosynthetic bradyrhizobia
Article first published online: 29 OCT 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Symbiosis. Editors: Professors Paola Bonfante, Karen Visick, and Moriya Ohkuma
Volume 12, Issue 8, pages 2152–2164, August 2010
How to Cite
Miché, L., Moulin, L., Chaintreuil, C., Contreras-Jimenez, J. L., Munive-Hernández, J.-A., Del Carmen Villegas-Hernandez, M., Crozier, F. and Béna, G. (2010), Diversity analyses of Aeschynomene symbionts in Tropical Africa and Central America reveal that nod-independent stem nodulation is not restricted to photosynthetic bradyrhizobia. Environmental Microbiology, 12: 2152–2164. doi: 10.1111/j.1462-2920.2009.02090.x
- Issue published online: 4 AUG 2010
- Article first published online: 29 OCT 2009
- Received 3 February, 2009; accepted 10 September, 2009.
Fig. S1. Absorption spectra of Bradyrhizobium cells. Cells were grown on modified YM medium plates under continuous 730 nm light (black lines) or in the dark (grey lines) for 5–7 days. The absorption spectra of the colonies were directly recorded with a spectrophotometer (Giraud et al., 2000). When present, the A870 peak corresponds to the LHI–Bacteriochlorophyll complex. (A) Bradyrhizobium sp. ORS278; (B) Bradyrhizobium sp. STM3850; (C) Bradyrhizobium sp. STM3964.
Fig. S2. Southern hybridizations of Bradyrhizobium spp. genomic DNA with nodA probes. Composite dot-blot representative of three independent hybridizations with a mixture of nodA probes from non-photosynthetic (column 1) and photosynthetic (column 2) strains. CI-group of each strain is given in Table S1.
Fig. S3. Stem and root nodulation of A. indica by cluster XI bradyrhizobia. Bacterial inoculation was performed in nitrogen-free medium on 3-day-old A. indica seedlings (root inoculation) or 2-week-old plants (stem inoculation), in independent experiments. Nodule formation was checked 4 weeks after inoculation. (A and E) control plants; (B and F) inoculation with B. japonicum USDA 110; (C and G) inoculation with Bradyrhizobium sp. STM3846; (D and H) inoculation with Bradyrhizobium sp. ORS278. White arrows, adventious stem and secondary root primordia; black arrows, stem and root nodules. Scale bar = 1 cm.
Table S1. Origin, nodulation specificity, photosynthetic phenotype and recA sequence accession numbers of Bradyrhizobium strains used in this study.
Table S2. Primers used for DNA amplification and sequencing.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.