Present address: Institute for General Microbiology, Christian Albrechts University, Am Botanischen Garten 1-9, 24118 Kiel, Germany. This work is dedicated to Professor Dr R.K. Thauer on the occasion of his 70th birthday.
Reductive dehalogenation of brominated ethenes by Sulfurospirillum multivorans and Desulfitobacterium hafniense PCE-S
Article first published online: 3 NOV 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 2, pages 501–509, February 2010
How to Cite
Ye, L., Schilhabel, A., Bartram, S., Boland, W. and Diekert, G. (2010), Reductive dehalogenation of brominated ethenes by Sulfurospirillum multivorans and Desulfitobacterium hafniense PCE-S. Environmental Microbiology, 12: 501–509. doi: 10.1111/j.1462-2920.2009.02093.x
- Issue published online: 26 JAN 2010
- Article first published online: 3 NOV 2009
- Received 21 July, 2009; accepted 10 September, 2009.
Sulfurospirillum multivorans and Desulfitobacterium hafniense PCE-S are anaerobes that can utilize tetrachloroethene (PCE) as an electron acceptor in their energy metabolism. The end-product of PCE reduction for both organisms is cis-1,2-dichloroethene, which is formed via trichloroethene as the intermediate. The bacteria were able to dehalogenate cis- and trans-1,2-dibromoethene (cDBE and tDBE) in growing cultures and cell extracts. Dibromoethene supported growth of both organisms. The organisms debrominated cDBE and tDBE to vinyl bromide (VB); D. hafniense PCE-S also produced ethene in addition to VB. The PCE reductive dehalogenases (PCE dehalogenases) of S. multivorans and D. hafniense PCE-S mediated the debromination of tribromoethene (TBE) and both isomers of 1,2-DBE, indicating that this enzyme was responsible for the reductive dehalogenation of brominated ethenes. cDBE, tDBE, 1,1-DBE and VB were formed upon TBE debromination; VB was the major end-product. The PCE dehalogenase of D. hafniense PCE-S also formed ethene. With the purified enzymes from both organisms the kinetic properties of dehalogenation of brominated alkenes were studied and compared with those of their chlorinated analogues.