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Fig. S1. Parsimony network of host cytochrome b genes. Parsimony network inferred from 484 positions of the cytochrome b gene of Rimicaris exoculata from Rainbow (red), TAG (green), Logatchev (orange) and South MAR (blue). Each circle represents one sequence (individual number listed in circles), except for Individuals 1 and 12, which were identical. Lines connecting genotypes are one nucleotide difference, small white circles represent hypothetical unsampled ancestors. Calculated with the TCS program (Clement et al., 2000).

Fig. S2. A. Fluorescence in situ hybridization of R. exoculata Individual 1 from the Rainbow vent field with the Epsilon 1 probe (pink) showing the abundance of the Epsilon 1 symbiont. The Gamma symbiont is shown in green (hybridized with the probe Rexogam1268RT).

B. FISH of the same individual with the Epsilon 3 probe (pink) showing the abundance of the Epsilon 4 symbiont. The Gamma symbiont is shown as above.

C. FISH of the same individual with the RexoepsLG86 (5′-ctcgtcagccagtgc-3′) with competitor (5′-ctcgtcagccagtac-3′), showing that the signals in (B) are due to the presence of the Epsilon 4 symbiont, and not the Logatchev Epsilon 3. DAPI stain is shown in blue in all 3 images. The scale bar in (C) (20 μm) applies to all images.

Table S1. Summary of sampling sites and cruises.

Table S2. FISH probes designed for this study.

Table S3. Individual numbers used for sampling, and corresponding name used in text and figures.

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EMI_2129_sm_Figure_legend.doc27KSupporting info item
EMI_2129_sm_Fig_S1.tif170KSupporting info item
EMI_2129_sm_Fig_S2.tif6702KSupporting info item
EMI_2129_sm_Table_S1-S3.doc117KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.