The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas
Article first published online: 13 JAN 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 4, pages 951–963, April 2010
How to Cite
Duperthuy, M., Binesse, J., Le Roux, F., Romestand, B., Caro, A., Got, P., Givaudan, A., Mazel, D., Bachère, E. and Destoumieux-Garzón, D. (2010), The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas. Environmental Microbiology, 12: 951–963. doi: 10.1111/j.1462-2920.2009.02138.x
- Issue published online: 29 MAR 2010
- Article first published online: 13 JAN 2010
- Received 11 August, 2009; accepted 23 November, 2009.
Fig. S1. Genetic organization of the ompU gene region. The region flanking ompU localized on chromosome 1 of V. splendidus LGP32 is compared with the corresponding region on the chromosome 1 of V. harveyi ATCC BAA-1116 and V. nigripulchritudo SFn1. Grey and black arrows are conserved in the three genetic regions. Dashed arrows refer to genes absent from the genome sequence context surrounding ompU in V. splendidus LGP32. Black arrows refer to genes with a potential role in resistance to antimicrobials/antibiotics. The gene names and labels are displayed above the arrows.
Fig. S2. Standardization of oyster experimental infections by V. splendidus LGP32. A. Three groups of 30 oysters were injected with wild-type V. splendidus LGP32 at the following doses: 2 × 108 (closed boxes), 5 × 108 (closed triangles) or 2 × 109 (closed diamonds) cfu per animal. A control was performed by injection of sterile sea water (SSW, closed circles). Oyster mortalities were monitored over 4 days and Kaplan–Meier survival curves were generated. B. The wild-type V. splendidus LGP32 was injected to oysters at a dose of 5 × 108 cfu per animal. Groups of 30 oysters were monitored for 4 days after infection. Kaplan–Meier survival curves were generated for four independent survival experiments. A different open symbol was attributed to every of the four replicates.
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