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Fig. S1. σ70-dependent transcription of the aer1 and aer3 operons. Luciferase activities of KT2440 (white bars) or a KT2440 derivative, PP2062, that overexpresses σ70 through a duplicate copy of the rpoD gene under the control of the araC/PBAD promoter (grey bars) carrying either the aer1 operon-luxAB reporter plasmid (pVI987) or the aer3 operon-luxAB reporter plasmid (pVI990) as indicated. Overexpression of σ70 was induced in the PP2062 strains by inclusion of 0.2% L-arabinose in the growth medium. Data are the average of duplicate determinations from two independent cultures assayed at late-exponential phase (E, OD600 ∼0.7), the exponential-to-stationary phase transition (T, OD600 ∼2.5), or stationary phase (S, OD600 ∼3.0), with exponential-phase output from the wild-type set as 1.

Table S1. Primers and linkers used in plasmid constructed in this study.

Table S2. pDM4-Km-based plasmids and primers used for inactivation of σ-factor encoding genes of P. putida KT2440.

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