The σ-factor FliA, ppGpp and DksA coordinate transcriptional control of the aer2 gene of Pseudomonas putida
Article first published online: 18 JAN 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Pseudomonas. Editors: Professors Burkhard Tummler, Victor de Lorenzo, Alain Filloux and Joyce Loper
Volume 12, Issue 6, pages 1439–1451, June 2010
How to Cite
Österberg, S., Skärfstad, E. and Shingler, V. (2010), The σ-factor FliA, ppGpp and DksA coordinate transcriptional control of the aer2 gene of Pseudomonas putida. Environmental Microbiology, 12: 1439–1451. doi: 10.1111/j.1462-2920.2009.02139.x
- Issue published online: 3 JUN 2010
- Article first published online: 18 JAN 2010
- Received 15 October, 2009; accepted 24 November, 2009.
Fig. S1. σ70-dependent transcription of the aer1 and aer3 operons. Luciferase activities of KT2440 (white bars) or a KT2440 derivative, PP2062, that overexpresses σ70 through a duplicate copy of the rpoD gene under the control of the araC/PBAD promoter (grey bars) carrying either the aer1 operon-luxAB reporter plasmid (pVI987) or the aer3 operon-luxAB reporter plasmid (pVI990) as indicated. Overexpression of σ70 was induced in the PP2062 strains by inclusion of 0.2% L-arabinose in the growth medium. Data are the average of duplicate determinations from two independent cultures assayed at late-exponential phase (E, OD600 ∼0.7), the exponential-to-stationary phase transition (T, OD600 ∼2.5), or stationary phase (S, OD600 ∼3.0), with exponential-phase output from the wild-type set as 1.
Table S1. Primers and linkers used in plasmid constructed in this study.
Table S2. pDM4-Km-based plasmids and primers used for inactivation of σ-factor encoding genes of P. putida KT2440.
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