Effect of the herbicide glyphosate on glyphosate-tolerant maize rhizobacterial communities: a comparison with pre-emergency applied herbicide consisting of a combination of acetochlor and terbuthylazine
Article first published online: 26 JAN 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 4, pages 1021–1030, April 2010
How to Cite
Barriuso, J., Marín, S. and Mellado, R. P. (2010), Effect of the herbicide glyphosate on glyphosate-tolerant maize rhizobacterial communities: a comparison with pre-emergency applied herbicide consisting of a combination of acetochlor and terbuthylazine. Environmental Microbiology, 12: 1021–1030. doi: 10.1111/j.1462-2920.2009.02146.x
- Issue published online: 29 MAR 2010
- Article first published online: 26 JAN 2010
- Received 13 August, 2009; accepted 26 November, 2009.
A comparison was made of the effect of glyphosate (Roundup®Plus), a post-emergency applied herbicide, and of Harness®GTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplification, cloning and sequencing of soil DNA encoding 16S rRNA, rhizobacterial DNA hybridization to commercially available genome-wide microarrays from the soil bacterium Streptomyces coelicolor, and high-throughput DNA pyrosequencing of the bacterial DNA coding for 16S rRNA hypervariable V6 region. The results obtained strongly suggest that both herbicides do in fact affect the maize rhizobacterial communities, glyphosate being, to a great extent, the environmentally less aggressive herbicide.