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Fig. S1. Protein composition of Magnetospirillum strain J10 influenced by growth conditions. Whole cell protein separated on SDS-PAGE of cells grown with acetate (heterotrophic, H), acetate and sulfide (mixotrophic, M), sulfide or thiosulfate (autotrophic, A) in continuous culture at D = 0.07 h−1. Arrows indicate changes in the banding pattern with different growth conditions. Green arrows indicate proteins that are differently expressed in acetate-oxidizing conditions (a) and orange arrows indicate proteins expressed in growth conditions with sulfide or thiosulfate (s). SDS-PAGE was performed using a 4% stacking and 10% resolving gel and each lane was loaded with 20 µg of protein in sample buffer. Protein was stained using Coomassie Blue.

Table S1. Neutrophilic, mesophilic sulfur-oxidizing bacteria affiliated to the Proteobacteria for which autotrophic growth yield and maximum growth rate have been determined. Observed yield (Ysx, g dry weight mol−1 thiosulfate; not corrected per mol dissimilatory substrate) and maximum growth rate (µmax, h−1) observed at 25–30°C. Also indicated is the affiliation to the class of the phylum Proteobacteria.

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