Reduced inorganic sulfur oxidation supports autotrophic and mixotrophic growth of Magnetospirillum strain J10 and Magnetospirillum gryphiswaldense
Version of Record online: 26 JAN 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 4, pages 1031–1040, April 2010
How to Cite
Geelhoed, J. S., Kleerebezem, R., Sorokin, D. Y., Stams, A. J. M. and Van Loosdrecht, M. C. M. (2010), Reduced inorganic sulfur oxidation supports autotrophic and mixotrophic growth of Magnetospirillum strain J10 and Magnetospirillum gryphiswaldense. Environmental Microbiology, 12: 1031–1040. doi: 10.1111/j.1462-2920.2009.02148.x
- Issue online: 29 MAR 2010
- Version of Record online: 26 JAN 2010
- Received 5 June, 2009; accepted 30 November, 2009.
Fig. S1. Protein composition of Magnetospirillum strain J10 influenced by growth conditions. Whole cell protein separated on SDS-PAGE of cells grown with acetate (heterotrophic, H), acetate and sulfide (mixotrophic, M), sulfide or thiosulfate (autotrophic, A) in continuous culture at D = 0.07 h−1. Arrows indicate changes in the banding pattern with different growth conditions. Green arrows indicate proteins that are differently expressed in acetate-oxidizing conditions (a) and orange arrows indicate proteins expressed in growth conditions with sulfide or thiosulfate (s). SDS-PAGE was performed using a 4% stacking and 10% resolving gel and each lane was loaded with 20 µg of protein in sample buffer. Protein was stained using Coomassie Blue.
Table S1. Neutrophilic, mesophilic sulfur-oxidizing bacteria affiliated to the Proteobacteria for which autotrophic growth yield and maximum growth rate have been determined. Observed yield (Ysx, g dry weight mol−1 thiosulfate; not corrected per mol dissimilatory substrate) and maximum growth rate (µmax, h−1) observed at 25–30°C. Also indicated is the affiliation to the class of the phylum Proteobacteria.
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