Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library
Version of Record online: 11 FEB 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Pseudomonas. Editors: Professors Burkhard Tummler, Victor de Lorenzo, Alain Filloux and Joyce Loper
Volume 12, Issue 6, pages 1468–1485, June 2010
How to Cite
Molina-Henares, M. A., De La Torre, J., García-Salamanca, A., Molina-Henares, A. J., Herrera, M. C., Ramos, J. L. and Duque, E. (2010), Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome-wide mutant library. Environmental Microbiology, 12: 1468–1485. doi: 10.1111/j.1462-2920.2010.02166.x
- Issue online: 3 JUN 2010
- Version of Record online: 11 FEB 2010
- Received 29 October, 2009; accept 15 December, 2009.
Fig. S1. Gene order of the grpE, dnaK, dnaJ, dapB and carA gene cluster. The gene order and the intergenic distance are indicated. dapB is co-transcribed with the three upstream genes, but not with the downstream carA gene.
Fig. S2. Metabolic pathway from glutamate yielding arginine and proline. Available mutants are described in the main text.
Fig. S3. (A) Gene cluster showing the argA/argE genes. (B) Electrophoresis of cDNA ampiflified with primers arg-1/arg-2. Lane 2 corresponds to the negative control and lane corresponds to the positive control. M refers to molecular weight marker.
Fig. S4. Biosynthetic pathway of histidine in KT2440. Pathway genes and functions were deduced from the original genome annotation (Nelson et al., 2002).
Fig. S5. Biosynthesis available mutants are described in the main text of asparagine, threonine, isoleucine, methionine and lysine from L-aspartate.
Fig. S6. Biosynthesis of L-cysteine, L-serine and L-glycine from 3-phosphoglycerate. Available mutants are described in the main text.
Fig. S7. Proposed pathway for the biosynthesis of aromatic amino acids phenylalanine, tyrosine and tryptophan in P. putida KT2440.
Fig. S8. Biosynthetic pathway of vitamin B12 in aerobic microorganisms.
Fig. S9. Gene organization of the clusters encoding the enzymes for cobalamin biosynthesis in P. putida KT2440, as deduced by Nelson and colleagues (2002).
Fig. S10. Proposed pathway for the biosynthesis of nicotinic acid in P. putida KT2440, as deduced from Nelson and colleagues (2002).
Table S1. a. Conditionally essential gene products by Puchalka and colleagues (2008). The corresponding PP number and the function in which the protein is are proposed to be involved in the metabolism of nucleotides, cofactors and cell processes is indicated. b. Conditionally essential genes as proposed by Nogales and colleagues (2008). The corresponding PP number and the function in which the protein is potentially involved are indicated. c. Conditionally essential genes as defined by iJN746 and iJP815.
Table S2. Eighteen broad-range substrate-specificity transaminases encoded in P. putida KT2440 genome.
Table S3. ORF annotated as genes encoding potential racemases.
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