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Ironing out the wrinkles in the rare biosphere through improved OTU clustering
Version of Record online: 11 MAR 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 7, pages 1889–1898, July 2010
How to Cite
Huse, S. M., Welch, D. M., Morrison, H. G. and Sogin, M. L. (2010), Ironing out the wrinkles in the rare biosphere through improved OTU clustering. Environmental Microbiology, 12: 1889–1898. doi: 10.1111/j.1462-2920.2010.02193.x
- Issue online: 5 JUL 2010
- Version of Record online: 11 MAR 2010
- Received 1 December, 2009; accepted 19 January, 2010.
Fig. S1. Reads with errors: observed vs. expected. We plotted the number of reads with one or more errors that we observed and that would be expected using a binomial distribution of errors. The binomial distribution underestimates the number of reads with multiple errors. The error rates used for each community are reported in Supplementary Table 2.
Table S1. Sequences generated from each clone experiment.
Table S2. Pyrosequencing and PCR error.
Table S3. PyroNoise comparison.
Table S4. Comparison of clustering methods after single-linkage preclustering (SLP).
Table S5. Amplification primers.
Table S6. Multiplexing barcodes used to differentiate subsamples of the two genomic template samples.
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