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Fig. S1. Growth (A540) of: (A) P. putida pMC (?), the mutant P. putida ΔtynA::Tn5 (?), P. putida ΔtynA::Tn5 pMC (?) and the recombinant strain P. putida ΔtynA::Tn5 pMCtynA (?); (B) P. putida pMC (?), P.putida ΔtynB::Tn5 (?), P. putida ΔtynB::Tn5 pMCtynB (?) and P. putida ΔtynB::pK18::mob (?); (C) P. putida ΔtynB::Tn5 pMCtynA (?), P. putida ΔtynB::Tn5 pMCtynB (?) and P. putida ΔtynB::Tn5 pMCtynAB (?) when cultured in MM containing tyramine (5 mM) as the sole carbon source. Similar results were obtained with dopamine (5 mM).

Fig. S2. Growth (A540) of P. putida pK18::mob (?) and the mutants P. putida ΔtynC::pK18::mob (?) and P. putida ΔtynR::pK18::mob (?) when cultured in MM containing tyramine (A) or 4HPA (B) as the sole carbon source (5 mM). Similar results were obtained with dopamine (5 mM).

Fig. S3. A. Growth (A540) of P. putida U (?) and a class 2 mutant (?), when cultured in MM containing tyramine (5 mM) and phenylacetic acid (PA) (5 mM).

B. Growth (A540) of P. putida U (?) and a class 3 mutant (ΔhpaD:Tn5) (?) when cultured in MM containing 4HPA (5 mM) and phenylacetic acid (PA) (5 mM). Residual concentrations of carbon sources and their catabolites (tyramine, red; 4HPA, blue; PA, green and 3,4HPA, yellow) are also indicated.

Fig. S4. Growth (A540) of P. putida U (?) and P. putida U ΔhpaX:Tn5 (?) when cultured in MM containing tyramine (A) or 4HPA (B) as the sole carbon source (5 mM). Residual concentration of the tyramine (red) or 4HPA (blue) when P. putida U (wild-type) and P. putida U ΔhpaX:Tn5 were cultured in the above conditions. In some cases, the detection of 4HPA is also indicated.

Fig. S5. Organization of the gene clusters encoding the 4HPA catabolic pathway in P. putida U and in different microbes. Colours correspond to genes encoding proteins with a similar function: red (regulators); blue (transporters); green (genes encoding the hydroxylase of HPA); yellow (the gene responsible for aromatic ring cleavage) and purple (those belonging to the meta pathway). The sequences of the different genes were obtained from the Genome Projects and carried the accession numbers: E. coli W, Z37980; Salmonella typhimurium, AM991977.1; Klebsiella pneumoniae, NC009648, Yersinia pestis, NC003143.1; Pseudomonas aeruginosa PAO1, NC002516.2 and P. entomophila L48, NC008027.1.

Fig. S6. Schematic representation of the procedure used to identify the chromosomal DNA fragments adjacent to the Tn5 arms in the P. putida U mutants restricted in their ability to degrade tyramine or dopamine. The DNA fragment adjacent to the transposon can be obtained by enzymatic digestion of the bacterial DNA with any of the following restriction enzymes: BamHI, XbaI, SmaI and SalI.

Table S1. Strains, mutants and vectors used in this work.

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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.