Lack of CbrB in Pseudomonas putida affects not only amino acids metabolism but also different stress responses and biofilm development
Article first published online: 7 JUN 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Pseudomonas. Editors: Professors Burkhard Tummler, Victor de Lorenzo, Alain Filloux and Joyce Loper
Volume 12, Issue 6, pages 1748–1761, June 2010
How to Cite
Amador, C. I., Canosa, I., Govantes, F. and Santero, E. (2010), Lack of CbrB in Pseudomonas putida affects not only amino acids metabolism but also different stress responses and biofilm development. Environmental Microbiology, 12: 1748–1761. doi: 10.1111/j.1462-2920.2010.02254.x
- Issue published online: 7 JUN 2010
- Article first published online: 7 JUN 2010
- Received 12 November, 2009; accepted 20 April, 2010.
Fig. S1. Growth of Pseudomonas putida KT2442 and the cbrB, ntrC and cbrBntrC mutant on different compounds. Cells were pregrown overnight on M9 medium (see Experimental procedures) and then transferred to M9 minimal medium supplemented with 20 mM amino acids as the sole carbon and/or nitrogen sources or citrate 20 mM as sole carbon source plus 20 mM ammoniun as nitrogen source. Cells were pregrown on LB media when growth on LB medium was tested. The mean values are average of three to eight data given by independent cultures.
Fig. S2. Flgaella of P. putida KT2442 wild type and cbrB mutant (MPO406) visualized after staining with crystal violet, by (A) confocal laser scanning and (B) phase contrast microscopy. (C) shows the anti-FilC inmunofluorescence labelling, where bacteria were fixed and the chromosome was stained with Hoechst 33258 (blue). Flagella were labelled with anti-FliC antiserum as described in Experimental procedures and detected with Alexa 568-conjugated goat anti-rabbit lgG conjugated (red). Pseudomonas aeruginosa PAO1 strain was used as positive control and P. putida fleQ mutant strain as negative control for PAO1 anti-FliC antibody (data not shown).
Table S1. Biolog phenotypic microarray data. Differences in the areas plotting redox activity monitored for 24 h between the wild type and cbrB mutant strains are quantitated as scores (see Experimental procedures). PM1 and PM2 are Carbon microplates, PM3, PM6 and PM7 are Nitrogen microplates, containing PM6 and PM7 peptides as nitrogen source.
Table S2. Microarray data for arrays 1 and 2. Fold change, P-value, adjusted P-value (Benjamini & Hochberg) and the average signal of six to eight replicas are shown (see Experimental procedures). Values over the cut-off set for both experiments (fold change > 1.8 or < −1.8 and P-value < 0.05) are only displayed.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.