Fig. S1. Multiple alignment of the nucleotide sequences of SfT6 and deduced S. frugiperda trypsin-like genes from the SPODOBASE ESTs clusters sf1f04035-3-1 and sf1f02950-3-1. The alignment only comprised the SfT6 region used for dsRNA generation.

Fig. S2. qRT-PCR analysis of mRNA levels from different S. frugiperda trypsins in untreated and dsRNA-SfT6 fed larvae. For each determination, midgut total RNA (100 ng) was isolated from a pool of 10 dissected larvae. The detected transcript amounts were normalized to the reference gene β-actin with the aid of Q-gene software. Bars represent means of two independent replicates ± SE.

EMI_2259_sm_fS1.pdf25KSupporting info item
EMI_2259_sm_fS2.doc34KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.