RNAi-mediated knockdown of a Spodoptera frugiperda trypsin-like serine-protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca1 protoxin
Version of Record online: 3 NOV 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 11, pages 2894–2903, November 2010
How to Cite
Rodríguez–Cabrera, L., Trujillo–Bacallao, D., Borrás–Hidalgo, O., Wright, D. J. and Ayra–Pardo, C. (2010), RNAi-mediated knockdown of a Spodoptera frugiperda trypsin-like serine-protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca1 protoxin. Environmental Microbiology, 12: 2894–2903. doi: 10.1111/j.1462-2920.2010.02259.x
- Issue online: 3 NOV 2010
- Version of Record online: 3 NOV 2010
- Received 19 October, 2009; accepted 12 April, 2010.
Fig. S1. Multiple alignment of the nucleotide sequences of SfT6 and deduced S. frugiperda trypsin-like genes from the SPODOBASE ESTs clusters sf1f04035-3-1 and sf1f02950-3-1. The alignment only comprised the SfT6 region used for dsRNA generation.
Fig. S2. qRT-PCR analysis of mRNA levels from different S. frugiperda trypsins in untreated and dsRNA-SfT6 fed larvae. For each determination, midgut total RNA (100 ng) was isolated from a pool of 10 dissected larvae. The detected transcript amounts were normalized to the reference gene β-actin with the aid of Q-gene software. Bars represent means of two independent replicates ± SE.
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