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Fig. S1. Unweighted pair group method using arithmetic averages (UPGMA) cluster analysis of DGGE patterns based on a band-based Dice similarity matrix. UPGMA comparison of DGGE patterns from short-term in situ and long-term laboratory incubations performed in 2005 and 2006. Left to right: UPGMA clustering of DGGE fingerprints. DGGE fingerprints, 48–63% denaturing gradient, as shown in Fig. 4. Dice similarity matrix including values used for calculation of the UPGMA dendrogram. C-L and C-D, light and dark control incubations; RB-L, incubation with increased [1O2]SS; RB-D, dark control for RB incubations.

Table S1. Limnological parameters of Lake Grosse Fuchskuhle (SW basin).

Table S2. Bacterial numbers (× 106) of in situ and laboratory incubations. Bacteria were counted after SYBR Green I staining. The fraction > 0.35 represents bacterial cells with a length larger than 0.35 μm and the fraction < 0.35 cells smaller than 0.35 μm in diameter (micrococcoids). The fraction of micrococcoid cells is additionally shown as per cent (%) of the total cell counts. C, control; RB, 0.2 μM Rose Bengal; L, light incubation; D, dark incubation. All values are means of cell counts obtained from 10 microscopic pictures obtained from two biological replicates with standard deviations given in brackets.

Table S3. Phylogenetic affiliation of 16S rRNA gene sequences representing DNA bands. Closest related 16S rRNA gene sequences have been determined by blast (Altschul et al., 1997). FSW, Grosse Fuchskuhle southwest basin; St, short-term in situ experiment; Lt, long-term laboratory experiment; 05, experiments performed 2005; 06, experiments performed 2006.

FilenameFormatSizeDescription
EMI_2285_sm_fS1.tif1640KSupporting info item
EMI_2285_sm_tS1-2.doc67KSupporting info item
EMI_2285_sm_tS3.doc182KSupporting info item

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