Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol
Article first published online: 21 JUL 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 12, Issue 12, pages 3253–3263, December 2010
How to Cite
Wharfe, E. S., Jarvis, R. M., Winder, C. L., Whiteley, A. S. and Goodacre, R. (2010), Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol. Environmental Microbiology, 12: 3253–3263. doi: 10.1111/j.1462-2920.2010.02300.x
- Issue published online: 3 DEC 2010
- Article first published online: 21 JUL 2010
- Received 15 October, 2009; accepted 7 June, 2010.
Fig. S1. FT-IR absorbance spectrum of pure potassium thiocyanate. An asterisk is used to highlight the main vibration feature at 2050 cm−1, which arises from S - C - N.
Fig. S2. FT-IR difference spectrum of: (average phenol IR spectrum at t - 48) minus (average control IR spectrum at t - 48). The peak highlighted with an asterisk is the carbonyl stretch at 1754.5–1710.2 cm−1 which increases during growth on phenol.
Fig. S3. Phenol quantification data (black symbols and lines) plotted with the COOH (1754.5–1710.2 cm−1); FT-IR peak areas shown in green. Data for all ageing experiments are shown (2, 9, 16 and 131 days; A, B, C and D respectively). Samples supplemented with 5 mM phenol are shown on the left hand side and samples grown in the absence of phenol (control) are on the right hand side.
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