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Fig. S1. Severity scale used to evaluate disease caused by P. fuscovaginae infection on Chenopodium quinoa: 0, no symptoms; 1, necrosis on less than 2 mm around the puncture; 2, necrosis on 2–10 mm around the puncture; 3, necrosis on 2–10 mm around the puncture and bending of the petiole; 4, collapse of the petiole; and 5, wilting of the leaf.

Fig. S2. Severity scale used to evaluate disease caused by P. fuscovaginae infection on rice: 0, no symptoms; 1, necrosis around the puncture till 1 cm; 2, necrosis around the puncture and chlorosis 1–3 cm on the new leaf; 3, necrosis around the puncture and chlorosis till 5 cm on the new leaf; 4, necrosis around the puncture and chlorosis for the two-thirds of the new leaf; and 5, necrosis around the puncture and chlorosis of all the new leaf.

Fig. S3. LC/MS/MS analysis of AHL synthetic standards and ethyl acetate extracted AHLs from P. fuscovaginae. The top two rows of every section refer to the reverse-phase chromatographic profile. AHLs were identified by retention time analysis and by comparison to synthetic standards. The third rows are the MS/MS fragmentation patterns. (A) C10-HSL synthetic standard; (B) C12-HSL synthetic standard; (C) 3-oxo-C10-HSL synthetic standard; (D) 3-oxo-C12-HSL synthetic standard; (E) UPB0736 wild-type extract, identification of C10-HSL; (F) UPB0736 wild-type extract, identification of C12-HSL; (G) UPB0736 wild-type extract, identification of 3-oxo-C10-HSL; (H) UPB0736 wild-type extract, identification of 3-oxo-C12-HSL; (I) extract of 0736IDM double pfvI and pfsI mutant harbouring pBBPfvI expressing pfvI, identification of 3-oxo-C10-HSL; (J) extract of 0736IDM double pfvI and pfsI mutant harbouring pBBPfvI expressing pfvI, identification of 3-oxo-C12-HSL; (K) extract of 0736IDM double pfvI and pfsI mutant harbouring pBBPfsI expressing pfsI, identification of C10-HSL; (L) extract of 0736IDM double pfvI and pfsI mutant harbouring pBBPfsI expressing pfsI, identification of C12-HSL; (M) extract of 0736PFVI single pfvI mutant, identification of C10-HSL; (N) extract of 0736PFVI single pfvI mutant, identification of C12-HSL; (O) extract of 0736PFSI single pfsI mutant, identification of 3-oxo-C10-HSL; (P) extract of 0736PFSI single pfsI mutant, identification of 3-oxo-C12-HSL.

Table S1. In this table, the ions monitored for each AHL in the mass spectrometric analyses are provided.

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FilenameFormatSizeDescription
EMI_2316_sm_fS1.tif23197KSupporting info item
EMI_2316_sm_fS2.tif29185KSupporting info item
EMI_2316_sm_fS3.ppt312KSupporting info item
EMI_2316_sm_tS1.doc24KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.