Fig. S1. Standard curve for quantification of eDNA concentration (mg g−1 VSS) in activated sludge. Relative intensity, describing the average ratio of DDAO fluorescence intensity to the biovolume of cells in a batch of at least 30 images, can be translated to the concentration of extracellular DNA in a given sample. The first point without eDNA concentration was obtained by digestion of eDNA with DNase I. The curve presented is an average of four consecutive experiments which provided very repetitive data. Error bars represent the standard error of the mean.

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