Extracellular DNA is abundant and important for microcolony strength in mixed microbial biofilms
Article first published online: 30 NOV 2010
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 13, Issue 3, pages 710–721, March 2011
How to Cite
Dominiak, D. M., Nielsen, J. L. and Nielsen, P. H. (2011), Extracellular DNA is abundant and important for microcolony strength in mixed microbial biofilms. Environmental Microbiology, 13: 710–721. doi: 10.1111/j.1462-2920.2010.02375.x
- Issue published online: 1 MAR 2011
- Article first published online: 30 NOV 2010
- Received 18 August, 2010; accepted 7 October, 2010.
Fig. S1. Standard curve for quantification of eDNA concentration (mg g−1 VSS) in activated sludge. Relative intensity, describing the average ratio of DDAO fluorescence intensity to the biovolume of cells in a batch of at least 30 images, can be translated to the concentration of extracellular DNA in a given sample. The first point without eDNA concentration was obtained by digestion of eDNA with DNase I. The curve presented is an average of four consecutive experiments which provided very repetitive data. Error bars represent the standard error of the mean.
|EMI_2375_sm_fS1.doc||61K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.