Cells dispersed from Marinobacter hydrocarbonoclasticus SP17 biofilm exhibit a specific protein profile associated with a higher ability to reinitiate biofilm development at the hexadecane–water interface

Authors

  • Pierre-Joseph Vaysse,

    1. Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, Equipe Environnement et Microbiologie, UMR5254 CNRS, IBEAS, Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
    Search for more papers by this author
  • Pierre Sivadon,

    1. Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, Equipe Environnement et Microbiologie, UMR5254 CNRS, IBEAS, Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
    Search for more papers by this author
  • Philippe Goulas,

    1. Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, Equipe Environnement et Microbiologie, UMR5254 CNRS, IBEAS, Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France.
    Search for more papers by this author
  • Régis Grimaud

    Corresponding authorSearch for more papers by this author

E-mail regis.grimaud@univ-pau.fr; Tel. (+33) 559 407 486; Fax (+33) 559 407 494.

Summary

Biofilm formation by marine hydrocarbonoclastic bacteria is commonly observed and has been recognized as an important mechanism for the biodegradation of hydrocarbons. In order to colonize new oil-water interfaces, surface-attached communities of hydrocarbonoclastic bacteria must release cells into the environment. Here we explored the physiology of cells freshly dispersed from a biofilm of Marinobacter hydrocarbonoclasticus developing at the hexadecane–water interface, by combining proteomic and physiological approaches. The comparison of the dispersed cells' proteome with those of biofilm, logarithmic- and stationary-phase planktonic cells indicated that dispersed cells had lost most of the biofilm phenotype and expressed a specific proteome. Two proteins involved in cell envelope maturation, DsbA and CtpA, were exclusively detected in dispersed cells, suggesting a reshaping of the cell envelopes during biofilm dispersal. Furthermore, dispersed cells exhibited a higher affinity for hexadecane and initiated more rapidly biofilm formation on hexadecane than the reference planktonic cells. Interestingly, storage wax esters were rapidly degraded in dispersed cells, suggesting that their observed physiological properties may rely on reserve mobilization. Thus, by promoting oil surface colonization, cells emigrating from the biofilm could contribute to the success of marine hydrocarbonoclastic bacteria in polluted environments.

Ancillary