Present address: Biocatalysis Unit, Catalysis Science Laboratory Mitsui Chemicals, Inc., 1144, Togo, Mobara-shi, Chiba, 297-0017, Japan;
Biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy
Article first published online: 7 MAR 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Thematic Issue: Extremophiles. Guest Editors: Ricardo Cavicchioli, Ricardo Amils, Dirk Wagner, Terry McGenity
Volume 13, Issue 8, pages 2092–2104, August 2011
How to Cite
Kimura, S., Bryan, C. G., Hallberg, K. B. and Johnson, D. B. (2011), Biodiversity and geochemistry of an extremely acidic, low-temperature subterranean environment sustained by chemolithotrophy. Environmental Microbiology, 13: 2092–2104. doi: 10.1111/j.1462-2920.2011.02434.x
- Issue published online: 21 AUG 2011
- Article first published online: 7 MAR 2011
- Received 22 September, 2010; accepted 6 January, 2011.
Fig. S1. Sampling sites within the abandoned Cae Coch mine: (A) mine roof (L1); (B) bacterial stalactite (arrowed, length ∼1.5 m; L2); and (C) the main stream flowing through the mine with acid streamer growths (stream width ∼0.5 m; L3); isolated pool (pH meter scale is 18 cm in height; L4).
Fig. S2. FISH analysis of: (A) and (C) an acid streamer (site L3M) and (B) a microbial stalactite (site L2) from Cae Coch. Micrographs (A1), (B1) and (C1) are strained with DAPI, and (A2), (B2) and (C2) with the fluorescein-labelled EUB338 probe. Sample (A3) is stained with the ‘Fv. myxofaciens’-specific probe BSC0459, sample (B3) with the Gallionella-like clone probe GALTS0084, and sample (C3) with the iron-oxidizing Acidithiobacillus probe TF539.
Fig. S3. FISH analysis of acid streamer and water samples from Cae Coch. (A) A streamer sample from site L3B stained with the fluorescein-labelled EUB338 probe (A1) and with the Cy3-labelled Sphingomonas-specific SPH120 probe (A2); (B) a streamer sample from site L3B stained with two probes targeting Firmicutes represented by clones L3B2C3 (B1) and L3B2C11 (B2); and (C) pool water sample L4B, targeted by the fluorescein-labelled EUB338 probe (C1) and the Acidimicrobium/Ferrimicrobium-specific probe ACM732 (C2).
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