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Fig. S1. Oxygen consumption measurement of whole cells. The oxygraph chamber was filled with air-saturated growth medium containing 20 mM glucose. When indicated by an arrow, 50 µl of washed cell suspension (0.2 mg total protein) was added. Experiment was performed at 37°C. One hundred per cent oxygen saturation corresponded to 211 µM dissolved oxygen at 37°C.

Fig. S2. UV-visible spectra of oxidized (plain line) and dithionite reduced (dotted line) recombinant NRO (A) and oxidized recombinant Rd (B). Insert: PAGE-SDS lane 1: the purified corresponding enzyme, lane 2: molecular weight markers.

Fig. S3. Temperature dependence of the NADH oxidoreductase-specific activity of NRO. The assay mixture contained 160 µM NADH and 1 µM NRO in air-saturated 50 mM MES buffer, pH 5.0.

Table S1. Table showing the taxonomic distribution of the homologues of rubrerythrin (Rbr, TM0657), neelaredoxin (Nlr, TM0658), rubredoxin (Rd, TM0659), NADH oxidoreductase (NRO, TM0754), flavo-diiron protein (FprA, TM0755) in the 1080 complete genomes analysed. Accessions numbers in the nr database are provided.

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EMI_2439_sm_fS1-S3.doc1949KSupporting info item
EMI_2439_sm_tS1.xls668KSupporting info item

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