Recipient of 2009 Humboldt Research Award. Mailing address: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. Fax: (+81) 77 438 3248; E-mail firstname.lastname@example.org.
Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures
Version of Record online: 25 APR 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Thematic Issue: Extremophiles. Guest Editors: Ricardo Cavicchioli, Ricardo Amils, Dirk Wagner, Terry McGenity
Volume 13, Issue 8, pages 2293–2298, August 2011
How to Cite
Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N. and Kurihara, T. (2011), Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures. Environmental Microbiology, 13: 2293–2298. doi: 10.1111/j.1462-2920.2011.02487.x
- Issue online: 21 AUG 2011
- Version of Record online: 25 APR 2011
- Received 16 March, 2011; accepted 16 March, 2011.
Fig. S1. Cell-size distribution of Shewanella violacea DSS12 and the EPA-less mutant at 30 MPa. The parent strain (S. violacea DSS12-rifampicin resistant; DSS12-Rif) and the EPA-less mutant (DSS12pfaA ) were cultured with the supplementation of EPA-containing phospholipid (sn-1-oleoly-sn-2-eicosapentaenoylphosphatidylethanolamine; OEPE) or phospholipid not containing EPA (sn-1,2-dioleoylphosphatidylethanolamine; DOPE) for 120 h at 6°C and 30 MPa.
Fig. S2. Immunofluorescence microscopy of Shewanella violacea DSS12 with anti-Sv FtsZ antibody. The parent strain, Shewanella violacea DSS12-rifampcin resistant, was cultured in Marine Broth 2216 at 30 MPa and fixed before pressure release.
A. Nucleoids stained with DAPI.
B. Immunofluorescence image with an anti-Sv FtsZ antibody of this strain.
C. Merged image.
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