Fig. S1. Cell-size distribution of Shewanella violacea DSS12 and the EPA-less mutant at 30 MPa. The parent strain (S. violacea DSS12-rifampicin resistant; DSS12-Rif) and the EPA-less mutant (DSS12pfaA ) were cultured with the supplementation of EPA-containing phospholipid (sn-1-oleoly-sn-2-eicosapentaenoylphosphatidylethanolamine; OEPE) or phospholipid not containing EPA (sn-1,2-dioleoylphosphatidylethanolamine; DOPE) for 120 h at 6°C and 30 MPa.

Fig. S2. Immunofluorescence microscopy of Shewanella violacea DSS12 with anti-Sv FtsZ antibody. The parent strain, Shewanella violacea DSS12-rifampcin resistant, was cultured in Marine Broth 2216 at 30 MPa and fixed before pressure release.

A. Nucleoids stained with DAPI.

B. Immunofluorescence image with an anti-Sv FtsZ antibody of this strain.

C. Merged image.

EMI_2487_sm_fS1.eps453KSupporting info item
EMI_2487_sm_fS2.tiff517KSupporting info item
EMI_2487_sm_suppl.doc32KSupporting info item

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