Fig. S1. Genome map of Desulfatibacillum alkenivorans AK-01. Innermost ring: G+C skew; second ring: G+C content; third ring: reverse CDS with COG designations; fourth ring: reverse strand; fifth ring: forward strand; outermost ring: forward CDS with COG designations.

Table S1. General features of the D. alkenivorans AK-01 genome.

Table S2. Alkane metabolism. (a) Alkylsuccinate synthase loci. (b) Comparison of iron-sulfur motifs in Bss, Ass and Mas proteins. (c) BLASTP matches of putative Ass locus 1 gene-encoded proteins.

Table S3. Beta-oxidation.

Table S4. Methylmalonyl-CoA pathway.

Table S5. Alcohol metabolism.

Table S6. Classified and unclassified 2-oxoacid ferredoxin oxidoreductases.

Table S7. Wood–Ljungdahl pathway.

Table S8. Glycolysis (Embden–Meyerhof–Parnas pathway) and gluconeogenesis.

Table S9. Pentose phosphate pathway.

Table S10. Entner–Doudoroff pathway (ED).

Table S11. Oxidation of pyruvate to acetyl-CoA and acetate formation.

Table S12. TCA cycle (oxidative).

Table S13. TCA cycle (reductive).

Table S14. Synthesis of oxaloacetate, fumarate and 2-oxoglutarate.

Table S15. Glyoxylate cycle.

Table S16. Fatty acid biosynthesis.

Table S17. Nitrogen metabolism.

Table S18. Sulfur metabolism.

Table S19. Response to oxygen.

Table S20. Membrane complexes.

Table S21. Periplasmic-facing formate dehydrogenases.

Table S22. Cytoplasmic formate dehydrogenases.

Table S23. Periplasmic-facing hydrogenases.

Table S24. Cytoplasmic hydrogenases.

Table S25. Multihaem cytochromes.

Table S26. Heterodisulfide reductases.

Table S27. Selenocysteine proteins and machinery.

Table S28. rRNAs and tRNAs.

Table S29. tRNA synthetases.

Tables S30 and S31. Motility and chemotaxis.

Table S30. Flagellum/chemotaxis.

Table S31. Pilus system.

Table S32. Transporters.

Table S33. Regulatory proteins (COG K).

Table S34. Analysis of intergenic regions.

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