Fig. S1. Phylogenetic compositions of archaeal clone libraries constructed from DNA and RNA extracted from sediments from (A) 11 m and (B) 20 m at the AHH site. Numbers of OTUs and clones (in parentheses) are shown for each phylogenetic group.

Fig. S2. Phylogenetic trees based on bacterial 16S rRNA gene sequences amplified from sediments from 11 and 20 m at the AHH site compared with reference strain sequences from GenBank. Trees indicate the phylogenetic relationship among (A) Proteobacteria and the related sequences, (B) Firmicutes, OP10, OP11 and related sequences, (C) Chloroflexi and JS1 and (D) Spirochaetes, Acidobacteria, Actinobacteria, Planctomycetes, Bacteroidetes, WS1, OP8 and related sequences. The tree was constructed using the neighbour-joining method with 658 bp of comparable sequences. Percentages greater than 50% of bootstrap resampling (of 1000 resamplings) that supported each topological element are indicated near the nodes. Sequences obtained in this study are in bold. The bar indicates the expected number of changes per sequence position.

Table S1. Distribution of the bacterial phylogenetic group in the DNA-based clone library constructed from sediments from 11 and 20 m at the AHH site. The number of OTUs and clones (in parentheses) are shown.

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