SEARCH

SEARCH BY CITATION

Fig. S1. Dependence of the conjugation rate γRecipient on growth tone α. The growth dependency cases 3 (left panel) and 7 (right panel) are shown as examples. γRecipient is calculated using equation (3) for the vs functions overlaid in the dashed plots.

Fig. S2. Output metrics for different model parameter variations. For each parameter set, the mean and standard deviation of 5 replicate simulations are plotted. Plots on the left show the plasmid success metric Sp, which measures the proportion of newly-created cells that contain a plasmid, and on the right the invasiveness metric RInvasion , which measures the degree to which the plasmid has invaded the original population. The rows illustrate these output metrics for different parameter variation sets listed in Table S5, with each output plotted against the value of the proficiency used. The constant-γ cases show variation by approximately a factor of two, while the constant product p·vs sets show variation by only a few percent.

Fig. S3. Contribution of HGT to overall plasmid spread as a function of growth dependence case. This metric shows the fraction of plasmid-bearing agents that received their plasmid through horizontal transfer rather than vertical transfer. Each bar represents the mean and standard deviation of 5 replicate simulations for the growth dependence cases from Figure 4 in the main text.

Fig. S4. Contribution of HGT to overall plasmid spread for young (left) and old (right) biofilms. This metric shows the fraction of plasmid-bearing agents that received their plasmid through horizontal transfer rather than vertical transfer. Each bar represents the mean and standard deviation of 5 replicate simulations for the invasion studies from Figures 11–12 in the main text.

Table S1. Stoichiometric matrix for default reactions.

Table S2. Stoichiometric matrix for plasmid-coded reactions.

Table S3. Growth and system parameter values.

Table S4. Parameters for dynamic bulk simulations.

Table S5. Parameter value variations for sensitivity analysis and different growth dependence cases.

Algorithm S1. Pseudo-code for a single global time-step.

Time series of plasmid invasion in old or young biofilms inoculated in three different positions (deep, medium, shallow) as a function of growth dependency cases 1, 3, and 4.

Movie S1. Old deep case 1.

Movie S2. Old deep case 3.

Movie S3. Old deep case 4.

Movie S4. Old medium case 1.

Movie S5. Old medium case 3.

Movie S6. Old medium case 4.

Movie S7. Old shallow case 1.

Movie S8. Old shallow case 3.

Movie S9. Old shallow case 4.

Movie S10. Young deep case 1.

Movie S11. Young deep case 3.

Movie S12. Young deep case 4.

Movie S13. Young medium case 1.

Movie S14. Young medium case 3.

Movie S15. Young medium case 4.

Movie S16. Young shallow case 1.

Movie S17. Young shallow case 3.

Movie S18. Young shallow case 4.

FilenameFormatSizeDescription
EMI_2535_sm_Fig_S1-4_Tab_S1-5_Algor_S1.pdf1126KSupporting info item
EMI_2535_sm_mS1.mpg458KSupporting info item
EMI_2535_sm_mS2.mpg458KSupporting info item
EMI_2535_sm_mS3.mpg460KSupporting info item
EMI_2535_sm_mS4.mpg454KSupporting info item
EMI_2535_sm_mS5.mpg458KSupporting info item
EMI_2535_sm_mS6.mpg458KSupporting info item
EMI_2535_sm_mS7.mpg458KSupporting info item
EMI_2535_sm_mS8.mpg456KSupporting info item
EMI_2535_sm_mS9.mpg458KSupporting info item
EMI_2535_sm_mS10.mpg450KSupporting info item
EMI_2535_sm_mS11.mpg452KSupporting info item
EMI_2535_sm_mS12.mpg452KSupporting info item
EMI_2535_sm_mS13.mpg450KSupporting info item
EMI_2535_sm_mS14.mpg452KSupporting info item
EMI_2535_sm_mS15.mpg452KSupporting info item
EMI_2535_sm_mS16.mpg450KSupporting info item
EMI_2535_sm_mS17.mpg450KSupporting info item
EMI_2535_sm_mS18.mpg452KSupporting info item
EMI_2535_sm_statistical_analysis.xls466KSupporting info item
EMI_2535_sm_supp_movie_legend.doc23KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.