Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variable across five ecosystems
Article first published online: 1 SEP 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 13, Issue 10, pages 2778–2793, October 2011
How to Cite
Weber, C. F., Zak, D. R., Hungate, B. A., Jackson, R. B., Vilgalys, R., Evans, R. D., Schadt, C. W., Megonigal, J. P. and Kuske, C. R. (2011), Responses of soil cellulolytic fungal communities to elevated atmospheric CO2 are complex and variable across five ecosystems. Environmental Microbiology, 13: 2778–2793. doi: 10.1111/j.1462-2920.2011.02548.x
- Issue published online: 10 OCT 2011
- Article first published online: 1 SEP 2011
- Received 8 February, 2011; accepted 17 June, 2011.
Fig. S1. The average abundance of the 15 most abundant OTUs (binned at a distance of 0.10) present in the replicate cbhI libraries for each of the six sites surveyed (± SE). Number of replicate libraries averaged = 17 (creosote root zone), 17 (crust), 6 (aspen plantation), 12 (loblolly pine plantation), 6 (scrub oak/palmetto) and 10 (marsh). Number above the bars are OTU identifiers assigned in Mothur. Note difference in scale on the y-axes.
Table S1. Fungal isolates and sporocarps PCR screened for the presence of the cbhI gene. Sources of cultures are as follows: A, Andrea Porras-Alfaro, Western Illinois University, Macomb, IL; F, Fusarium Research Center, Department of Plant Pathology, Pennsylvania State University, State College, PA; C, Carolina Biological; T, Terri Porter, McMaster University, Hamilton, Ontario, Canada; USDA, United States Department of Agriculture; DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; IHEM, Belgian Co-ordinated collection of Microorganisms; NM, sporocarp collected in New Mexico; IL, sporocarp collected in Northern Illinois.
Table S2. OTU-based classification of soil sequences based on clustering with reference cbhI sequences from named taxa.
Table S3. Per cent composition based on phylum top BLAST hit of all 10 677 soil sequences.
Table S4. Phylum level classification (by top BLAST hit) of sequences that are unique to a given site.
Table S5. Soil properties in each of the treatment and control plots at the FACE and OTC sites surveyed in this study. Values represent an average of two replicates. Phosphorus (P) was NaHCO3 extracted.
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