The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c-di-GMP signalling
Version of Record online: 28 SEP 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Thematic Issue: Human Microbiome
Volume 13, Issue 12, pages 3128–3138, December 2011
How to Cite
Moscoso, J. A., Mikkelsen, H., Heeb, S., Williams, P. and Filloux, A. (2011), The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c-di-GMP signalling. Environmental Microbiology, 13: 3128–3138. doi: 10.1111/j.1462-2920.2011.02595.x
- Issue online: 30 NOV 2011
- Version of Record online: 28 SEP 2011
- Received 21 March 2011; accepted 15 August 2011.
Fig. S1. Overexpression of wspR or wspRR242A influences biofilm-related phenotypes. PAK wild type carrying either empty vector or overexpressing wspR or wspRR242A , as indicated in (A). A. Biofilm formation in static growth conditions visualized by crystal violet staining. B. Biofilm formation in shaking growth conditions quantified by crystal violet staining. C. Bacterial colony on Congo red agar. D. Swimming motility determined in 0.3% agar.
Fig. S2. Overproduction of a phosphodiesterase suppresses the hyperbiofilm phenotype of a retS mutant. Biofilm formation in glass tubes, visualized by crystal violet staining (left panel), and Congo red binding (right panel) of PAKΔretS carrying either empty vector or overproducing the phosphodiesterase PA2133.
Table S1. Strains and plasmids used in this study.
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