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Fig. S1. Contigs length distribution of Thetis interface metagenome.

Fig. S2. Contigs length distribution of Thetis brine metagenome.

Fig. S3. Bacterial phylogenetic tree [proteobacterial (A) and other clades (B)] based on the affiliation of 16S rRNA tag sequences retrieved from the Thetis interface (purple) and brine (blue) metagenomes and all 16S crRNA sequences recovered by sequence analysis of 16S crDNA clone libraries (La Cono et al., 2011) (green).

Fig. S4. Archaeal phylogenetic tree based on the affiliation of 16S rRNA tag sequences retrieved from the Thetis interface (purple) and brine (blue) metagenomes and all 16S crRNA sequences recovered by sequence analysis of 16S crDNA clone libraries (La Cono et al., 2011) (green).

Table S1. Annotation of genes relevant to the principal metabolic pathways in the brine and interface compartments of the Lake Thetis.

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