Present addresses: CNRS/UPMC Univ. Paris 06, UMR 7144, Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff, France;
Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses
Version of Record online: 9 JAN 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Ecology, Evolution and Population Genetics of Pathogenic Microbes
Volume 14, Issue 8, pages 2071–2086, August 2012
How to Cite
Baudoux, A.-C., Hendrix, R. W., Lander, G. C., Bailly, X., Podell, S., Paillard, C., Johnson, J. E., Potter, C. S., Carragher, B. and Azam, F. (2012), Genomic and functional analysis of Vibrio phage SIO-2 reveals novel insights into ecology and evolution of marine siphoviruses. Environmental Microbiology, 14: 2071–2086. doi: 10.1111/j.1462-2920.2011.02685.x
- Issue online: 26 JUL 2012
- Version of Record online: 9 JAN 2012
- Received 29 June, 2011; revised 15 November, 2011; accepted 27 November, 2011.
Fig. S1. Isolation of Vibrio phage SIO-2 from plaque assay.
Fig. S2. Vibrioid cell tagged with fluorescently labelled SIO-2 observed by epifluorescence microscopy.
Fig. S3. Genome size of SIO-2 isolate as determined by PFGE. Lane M: Lambda concatamers ladder, Lane 1: SIO-2 genome incubated with DNase RQ1 for 24 h, Lane 2: untreated SIO-2 genome.
Fig. S4. Intergenic repeats observed in SIO-2 genome. The termination codons of the upstream genes are shown in red, and the initiation codons of the downstream genes are shown in green. The five different repeated sequences are shown in five different colours. Putative transcription and translation signals indicated with labels and underlines include −35 and −10 regions of promoters (with the predicted + 1 nucleotide of the transcript indicated in bold), transcription terminators and Shine–Dalgarno ribosome-binding sequences.
Fig. S5. Codon usage in the genome of Vibrio sp. SWAT3 (H1) and Vibrio harveyi ATCC BAA-1116 (H2) and Vibrio phage SIO-2 (P).
Table S1. Bacterial cultures tested for the determination of SIO-2 host specificity. Efficiency of plating (EOP) was determined by the ratio of SIO-2 plaque titre obtained with the heterologous host to that obtained with Vibrio sp. SWAT3.
Table S2. Phage polymerase gene sequences (family A, PF00476.13) used for phylogenetic analysis.
Table S3. Metagenomic analysis.
Appendix S1. Material and methods.
|EMI_2685_sm_FigS1-5-TabS1-3-AppS1.doc||373K||Supporting info item|
|EMI_2685_sm_SIO2-Genome-sequence.doc||291K||Supporting info item|
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