Magnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1
Article first published online: 23 FEB 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Microbe:Metal Interactions
Volume 14, Issue 7, pages 1722–1729, July 2012
How to Cite
Guo, F. F., Yang, W., Jiang, W., Geng, S., Peng, T. and Li, J. L. (2012), Magnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1. Environmental Microbiology, 14: 1722–1729. doi: 10.1111/j.1462-2920.2012.02707.x
- Issue published online: 2 JUL 2012
- Article first published online: 23 FEB 2012
- Received 21 July, 2011; revised 16 January, 2012; accepted 20 January, 2012.
Fig. S1. The iron content in the cytosol of cell in mutant (Mu21-415), complemented strain (Mu21C) and wild type (WT-415). All M. gryphiswaldense strains (wild-type, mutants and complemented strains) were cultured in SLM I for 24 h until stationary phase. When the optical density at 600 nm reached 0.6, 0.3 mM IPTG was added to the culture of complemented strains. 1 l culture was centrifuged respectively at 7000 g for 5 min. The cells were washed by Tris-HCl buffer (20 mM, 4 mM EDTA, pH 7.4) three times and then resuspended by Tris-HCl buffer without EDTA (20 mM, pH 7.4). Suspension of cells (0.34 g ml−1) was disrupted by ultrasonic (200 w, 100 times) and centrifuged at 12 000 g for 20 min. The supernatant was used for ferric ion estimation. Ferric ion concentration was determined as described previously [Tamura H et al. Talanta 1974, 21(4):314–318] with modification as follows. To 100 μl of sample, 250 μl of 10% hydroxylamine hydrochloride and 20 μl 0.25% 1, 10-phenanthroline were added. After 15 min, the absorbance of sample solutions was determined spectrophotometrically at 562 nm.
Table S1. Bacterial strains used in this study.
|EMI_2707_sm_FigS1-TabS1.doc||36K||Supporting info item|
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