Pseudomonas aeruginosa population structure revisited under environmental focus: impact of water quality and phage pressure
Article first published online: 6 MAR 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Ecology, Evolution and Population Genetics of Pathogenic Microbes
Volume 14, Issue 8, pages 1952–1967, August 2012
How to Cite
Selezska, K., Kazmierczak, M., Müsken, M., Garbe, J., Schobert, M., Häussler, S., Wiehlmann, L., Rohde, C. and Sikorski, J. (2012), Pseudomonas aeruginosa population structure revisited under environmental focus: impact of water quality and phage pressure. Environmental Microbiology, 14: 1952–1967. doi: 10.1111/j.1462-2920.2012.02719.x
- Issue published online: 26 JUL 2012
- Article first published online: 6 MAR 2012
- Received 28 January, 2011; revised 3 February, 2012; accepted 8 February, 2012.
Pseudomonas aeruginosa attracts research attention as a common opportunistic nosocomial pathogen causing severe health problems in humans. Nevertheless, its primary habitat is the natural environment. Here, we relate the genetic diversity of 381 environmental isolates from rivers in northern Germany to ecological factors such as river system, season of sampling and different levels of water quality. From representatives of 99 environmental clones, also in comparison with 91 clinical isolates, we determined motility phenotypes, virulence factors, biofilm formation, serotype and the resistance to seven environmental P. aeruginosa phages. The integration of genetic, ecological and phenotypic data showed (i) the presence of several extended clonal complexes (ecc) which are non-uniformly distributed across different water qualities, and (ii) a correlation of the hosts' serotype composition with susceptibility towards distinct groups of environmental phages. For at least one ecc (eccB), we assumed the ecophysiological differences on environmental water adaptation and phage resistance to be so distinct as to reinforce an environmentally driven cladogenic split from the remainder of P. aeruginosa. In summary, we conclude that the majority of the microevolutionary population dynamics of P. aeruginosa were shaped by the natural environment and not by the clinical habitat.