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Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals
Article first published online: 16 APR 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Ecology, Evolution and Population Genetics of Pathogenic Microbes
Volume 14, Issue 8, pages 2184–2199, August 2012
How to Cite
Sweet, M. and Bythell, J. (2012), Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals. Environmental Microbiology, 14: 2184–2199. doi: 10.1111/j.1462-2920.2012.02746.x
- Issue published online: 26 JUL 2012
- Article first published online: 16 APR 2012
- Received 12 September, 2011; revised 9 March, 2012; accepted 16 March, 2012.
Vol. 14, Issue 12, 3288, Article first published online: 4 DEC 2012
White Syndrome (WS) and Brown Band Disease (BrB) are important causes of reef coral mortality for which causal agents have not been definitively identified. Here we use culture-independent molecular techniques (DGGE and clone libraries) to characterize ciliate and bacterial communities in these diseases. Bacterial (16S rRNA gene) and ciliate (18S rRNA gene) communities were highly similar between the two diseases. Four bacterial and nine ciliate ribotypes were observed in both diseases, but absent in non-diseased specimens. Only one of the bacteria, Arcobacter sp. (JF831360) increased substantially in relative 16S rRNA gene abundance and was consistently represented in all diseased samples. Four of the eleven ciliate morphotypes detected contained coral algal symbionts, indicative of the ingestion of coral tissues. In both WS and BrB, there were two ciliate morphotypes consistently represented in all disease lesion samples. Morph1 (JN626268) was observed to burrow into and underneath the coral tissues at the lesion boundary. Morph2 (JN626269), previously identified in BrB, appears to play a secondary, less invasive role in pathogenesis, but has a higher population density in BrB, giving rise to the visible brown band. The strong similarity in bacterial and ciliate community composition of these diseases suggests that they are actually the same syndrome.