Pronounced seasonal dynamics of freshwater chitinase genes and chitin processing
Article first published online: 7 MAY 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Microbial Communities - Structure, Behaviour, Evolution
Volume 14, Issue 9, pages 2467–2479, September 2012
How to Cite
Beier, S., Mohit, V., Ettema, T. J. G., Östman, Ö., Tranvik, L. J. and Bertilsson, S. (2012), Pronounced seasonal dynamics of freshwater chitinase genes and chitin processing. Environmental Microbiology, 14: 2467–2479. doi: 10.1111/j.1462-2920.2012.02764.x
- Issue published online: 4 SEP 2012
- Article first published online: 7 MAY 2012
- Received 22 October, 2011; revised 12 March, 2012; accepted 20 March, 2012.
Fig. S1. Number of chitinase reads that are necessary to keep the deviation of the Bray-Curtis distance below 0.05 if compared with the Bray-Curtis distance calculated from all available reads (‘true Bray-Curtis-distance’). 07-04-08H: high-diversity sample (chao1: 155); ss07-14-08H: high diversity sample (chao1: 180); 08-28-08: medium-diversity sample (chao1: 74).
Fig. S2. RAxML tree of chitinase genes (GH18) based on 100 bootstraps. Group A, B and C chitinase genes are indicated. Each OTU is represented by its longest sequence (OTU_0–OTU_399). Sequences representing the 15 most abundant OTUs are highlighted in colour. For non-bacterial reference sequences the domain is indicated.
Table S1. Used pyrosequencing barcodes and pyrosequencing output data (OTU: operational taxonomic unit).
Table S2. Arrangement of chitinase gene (GH18) reads into OTUs using CD-Hit program (75% cut-off level). An asterisk indicates the longest sequence of each OTU, which was used as reference sequence. For all other reads within an OTU similarities to this reference read are given.
Table S3. Species data. Relative frequency of the OTUs derived from pyrosequencing of chitinase genes (CD-Hit program, 75% cut-off level).
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