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Fig. S1. Light regime during the experiment. The light intensity was increased during phase 2, in order to promote carbon overconsumption, and thus DOC release. Sampling events are marked with black circles, and radiotracer incubations are indicated by white circles.

Fig. S2. Dynamics of particulate organic nitrogen (PON) and cellular carbon : nitrogen ratios (POC : PON) in the different treatments (1×–5×) over the duration of the experiment. According to the dynamics of the bloom development and based on productivity data, the experiment is divided into a net autotrophic phase 1 and a net heterotrophic phase 2. Each value represents six replicates and error bars indicate the standard error of the computed mean.

Fig. S3. Dynamics of nitrate (NO3-), nitrite (NO2-), ammonium (NH4+), dissolved organic nitrogen (DON), phosphate (PO43−) and silica (SiO4), dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC) in the different treatments during the experiment. Each symbol represents the mean value of six true replicates. Error bars indicate the standard error of the calculated mean.

Fig. S4. (A) Primary production (PP), (B) heterotrophic biomass production (Leu incorporation) and (C) bacterial cell division (Thy incorporation) during the different phases of the experiment. Each symbol represents the mean value of six true replicates. Blanks were always lower than 1% and were neglected. Error bars indicate the standard error of the calculated mean.

Fig. S5. (A) Abundance of phytoplankton and (B) bacteria, measured by Flow Cytometry (Accuri C6) daily in the different treatments (1×–5×) during the experiment. Each symbol represents the mean value of six true replicates. Error bars indicate the standard error of the calculated mean.

Table S1. Chemical and biological characterization of the field sample (before the addition of nitrate and phosphorous); mean = average of six independent replicates, sd = standard error of the calculated mean.

Table S2. Comparison of chemical and biological properties from downstream Childs River at different dates in 2009 and during this study.

FilenameFormatSizeDescription
emi2773_sm_FigS1-5-TabS1-2.doc62KSupporting info item
emi2773_sm_FigS1.tif198KSupporting info item
emi2773_sm_FigS2.tif198KSupporting info item
emi2773_sm_FigS3.tif315KSupporting info item
emi2773_sm_FigS4.tif224KSupporting info item
emi2773_sm_FigS5.tif203KSupporting info item

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