Water–sediment niche differentiation in ancient marine lineages of Exiguobacterium endemic to the Cuatro Cienegas Basin
Article first published online: 29 MAY 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Microbial Communities - Structure, Behaviour, Evolution
Volume 14, Issue 9, pages 2323–2333, September 2012
How to Cite
Rebollar, E. A., Avitia, M., Eguiarte, L. E., González-González, A., Mora, L., Bonilla-Rosso, G. and Souza, V. (2012), Water–sediment niche differentiation in ancient marine lineages of Exiguobacterium endemic to the Cuatro Cienegas Basin. Environmental Microbiology, 14: 2323–2333. doi: 10.1111/j.1462-2920.2012.02784.x
- Issue published online: 4 SEP 2012
- Article first published online: 29 MAY 2012
- Received 10 June, 2011; accepted 25 April, 2012.
Fig. S1. Principal component analysis of the ionic concentration in the CCB aquatic systems.
a. Eigenvalues and percentage of the variance explained by each of the principal components (PCs). Components in red are the ones with the highest eigenvalues and contribution of variance percentage.
b. Plot showing the coefficient values for the three PCs.
Fig. S2. Estimated means obtained by linear mixed models of the three principal components (PCs). X-axis shows the aquatic systems analysed (CH, RM, LH, PA). Y-axes show the estimated means of each principal component. Sediment and water are shown in black and white circles respectively. (a), (b) and (c) show the respective estimated mean values for PC1, PC2 and PC3 and their correspondent ions.
Fig. S3. Principal component analysis of the ionic concentration at the CCB aquatic systems and other saline environments worldwide (Oren, 2006). Top right table shows the eigenvalues and percentage variance explained by each of the principal components (PCs). Components in red are the ones with the highest eigenvalues and contribution of variance percentage. Plots show the four CCB sites (CH, RM, LH, PA) and the seven worldwide sites against the values for the three PCs in pairwise combinations. Blue and red labels mean water (w) and sediment (s) values from the CCB systems respectively. Seawater KCl means seawater at onset on KCl precipitation, and the same for seawater NaCl.
Table S1. Ionic composition of the water and sediment of each aquatic system. Top table shows the concentration of the cations and bottom table shows the concentration of the anions (average and standard deviation). Red numbers show the highest ionic values that differ by at least an order of magnitude.
Table S2. Genetic diversity of the phylogroups F1, F2 and F3 with five molecular markers. π is Nei's pairwise nucleotide diversity, θ is Waterson's theta per site = 2Neμ. Numbers in parenthesis show standard deviation values. H = number of haplotypes, S = number of segregant sites/total nucleotide number, Hd = haplotypic diversity. The two last columns show Tajima's D-values with its respective P-value. Red numbers show the significant values for this test (Rozas and Rozas, 1999).
Appendix S1. Supplementary methods.
|EMI_2784_sm_AppendixS1.doc||63K||Supporting info item|
|EMI_2784_sm_Figures.doc||190K||Supporting info item|
|EMI_2784_sm_Refs.doc||42K||Supporting info item|
|EMI_2784_sm_Tables.doc||112K||Supporting info item|
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