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Fig. S1. Principal component analysis of the ionic concentration in the CCB aquatic systems.

a. Eigenvalues and percentage of the variance explained by each of the principal components (PCs). Components in red are the ones with the highest eigenvalues and contribution of variance percentage.

b. Plot showing the coefficient values for the three PCs.

Fig. S2. Estimated means obtained by linear mixed models of the three principal components (PCs). X-axis shows the aquatic systems analysed (CH, RM, LH, PA). Y-axes show the estimated means of each principal component. Sediment and water are shown in black and white circles respectively. (a), (b) and (c) show the respective estimated mean values for PC1, PC2 and PC3 and their correspondent ions.

Fig. S3. Principal component analysis of the ionic concentration at the CCB aquatic systems and other saline environments worldwide (Oren, 2006). Top right table shows the eigenvalues and percentage variance explained by each of the principal components (PCs). Components in red are the ones with the highest eigenvalues and contribution of variance percentage. Plots show the four CCB sites (CH, RM, LH, PA) and the seven worldwide sites against the values for the three PCs in pairwise combinations. Blue and red labels mean water (w) and sediment (s) values from the CCB systems respectively. Seawater KCl means seawater at onset on KCl precipitation, and the same for seawater NaCl.

Table S1. Ionic composition of the water and sediment of each aquatic system. Top table shows the concentration of the cations and bottom table shows the concentration of the anions (average and standard deviation). Red numbers show the highest ionic values that differ by at least an order of magnitude.

Table S2. Genetic diversity of the phylogroups F1, F2 and F3 with five molecular markers. π is Nei's pairwise nucleotide diversity, θ is Waterson's theta per site = 2Neμ. Numbers in parenthesis show standard deviation values. H = number of haplotypes, S = number of segregant sites/total nucleotide number, Hd = haplotypic diversity. The two last columns show Tajima's D-values with its respective P-value. Red numbers show the significant values for this test (Rozas and Rozas, 1999).

Appendix S1. Supplementary methods.

FilenameFormatSizeDescription
EMI_2784_sm_AppendixS1.doc63KSupporting info item
EMI_2784_sm_Figures.doc190KSupporting info item
EMI_2784_sm_Refs.doc42KSupporting info item
EMI_2784_sm_Tables.doc112KSupporting info item

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