Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response
Article first published online: 5 JUN 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Baeza
Volume 15, Issue 1, pages 111–120, January 2013
How to Cite
Bobadilla Fazzini, R. A., Skindersoe, M. E., Bielecki, P., Puchałka, J., Givskov, M. and Martins dos Santos, V. A. P. (2013), Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response. Environmental Microbiology, 15: 111–120. doi: 10.1111/j.1462-2920.2012.02792.x
- Issue published online: 3 JAN 2013
- Article first published online: 5 JUN 2012
- Received 14 March, 2012; revised 27 April, 2012; accepted 4 May, 2012.
Many Gram-negative bacteria employ cell-to-cell communication mediated by N-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. Outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. We here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads Pseudomonas sp. B13 and Pseudomonas reinekei MT1 as a blind end in the biodegradation of organochloride xenobiotics, which inhibits quorum sensing in P. aeruginosa in naturally occurring concentrations. This catabolite, 4-methylenebut-2-en-4-olide, also known as protoanemonin, has been reported to possess antibacterial properties, but seems to have dual functions. Using transcriptomics and proteomics, we found that protoanemonin significantly reduced expression of genes and secretion of proteins known to be under control of quorum sensing in P. aeruginosa. Moreover, we found activation of genes and gene products involved in iron starvation response. It is thus likely that inhibition of quorum sensing, as the production of antibiotics, is a phenomenon found in complex bacterial communities.