Present address: CIRAD, UMR LSTM, F34398 Montpellier, France.
The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response
Version of Record online: 19 JUN 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Thematic issue: Sulfur Metabolism
Volume 14, Issue 10, pages 2645–2660, October 2012
How to Cite
Prigent-Combaret, C., Sanguin, H., Champier, L., Bertrand, C., Monnez, C., Colinon, C., Blaha, D., Ghigo, J.-M. and Cournoyer, B. (2012), The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response. Environmental Microbiology, 14: 2645–2660. doi: 10.1111/j.1462-2920.2012.02802.x
- Issue online: 3 OCT 2012
- Version of Record online: 19 JUN 2012
- Received 18 December, 2011; accepted 15 May, 2012.
Fig. S1.Escherichia coli EC3 colonies expressing the bTPMT and growing on LB agar supplemented with 126 µM K2TeO3. The shiny aspect of the colonies is illustrated by the presence of a translucide extracellular material (see arrow). Black Te crystals are also observed.
Fig. S2.Escherichia coli EC3 cells expressing the bTPMT and grown in LB broth supplemented with 40 µM K2TeO3.
A. Biofilm formation on the sides of a glass bottle used during the growth assays shown in Fig. 1.
B. Visualization of the cell aggregates of culture (A) after transfer in a petri dish. Inside each panel, controls are shown and represent the observations made from the same bacterial cells but not growing with a tellurite supplement. The scale bars indicate 10 mm.
Fig. S3. Effect of K2TeO3 and H2O2 on sessile bacterial concentrations of Escherichia coli EC3 cells grown in 24-well polystyrene plates using a modified LB broth supplemented with ampicillin and incubated 72 h at 37°C. Bacterial concentrations were estimated by OD600 readings but see the Experimental procedures section for further details. Data are expressed as means ± SD of the percentage of sessile bacteria over the total bacterial concentration of three independent samples.
Fig. S4. Total ion chromatogram of the gas chromatography-mass spectrometry (GC-MS) analysis of headspace gases of Escherichia coli EC3 cells grown in LB broth supplemented with 20 µM K2TeO3. This experiment is representative of three.
Table S1. Up- and downregulated gene categories per functional group at 20 µM K2TeO3.
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