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Fig. S1. Genetic organization of plasmid replication modules inserted within the photosynthesis gene clusters (PGC) of Roseobacter litoralis and Sulfitobacter guttiformis. Genes are coloured according to Fig. 3: light blue, parAB partitioning genes; yellow, dnaA-like replicase; pink, repB-III type replicase; white, genes specifically found in plasmid PGCs. Adjacent conserved genes of the PGC are shaded in grey. The origin of replication (ori) is indicated by a red circle and triangles represent palindromic sequences (inverted repeats) that may reflect cis-acting anchors for plasmid partitioning.

Fig. S2. Phylogenetic Maximum Likelihood tree of the plasmid partitioning protein ParB from Rhodobacterales based on 40 sequences and 216 amino acid positions, inferred by RAxML under an LG+F+Γ4 model. Sequences from Sulfitobacter guttiformis and Roseobacter litoralis are shown in bold. Coloured boxes reflect distinct plasmid compatibility groups of Rhodobacterales (yellow: DnaA-like I, orange: RepABC, blue: RepB-III). The statistical support for the internal nodes was determined by 100 bootstrap replicates and only values > 50% are shown. Accession numbers of all sequences are summarized in Table S1.

Fig. S3. Comparison between the conserved photosynthesis gene cluster (PGC) region of Roseobacter denitrificans and R. litoralis. Homology is indicated by vertical grey shaded areas; conserved genes of the PGC are coloured in pink, others in white. Plasmid-specific genes of the DnaA-like replication module and genes adjacent to the PGC are shown in yellow and red respectively. Adjacent chromosomal genes are indicated with grey lines. A pseudogene is coloured in grey and the origin of plasmid replication (ori) is indicated by a red circle.

Table S1. Accession numbers of replication and partitioning genes used for phylogenetic analyses (Figs 2, 4 and S2).

FilenameFormatSizeDescription
emi2806_sm_FS1.ppt28KSupporting info item
emi2806_sm_FS2.ppt70KSupporting info item
emi2806_sm_FS3.ppt71KSupporting info item
emi2806_sm_TS1.doc53KSupporting info item
emi2806_sm_TS2-3.doc40KSupporting info item

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