Ecological niche separation in the Polynucleobacter subclusters linked to quality of dissolved organic matter: a demonstration using a high sensitivity cultivation-based approach
Article first published online: 4 JUL 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Microbial Communities - Structure, Behaviour, Evolution
Volume 14, Issue 9, pages 2511–2525, September 2012
How to Cite
Watanabe, K., Komatsu, N., Kitamura, T., Ishii, Y., Park, H.-D., Miyata, R., Noda, N., Sekiguchi, Y., Satou, T., Watanabe, M., Yamamura, S., Imai, A. and Hayashi, S. (2012), Ecological niche separation in the Polynucleobacter subclusters linked to quality of dissolved organic matter: a demonstration using a high sensitivity cultivation-based approach. Environmental Microbiology, 14: 2511–2525. doi: 10.1111/j.1462-2920.2012.02815.x
- Issue published online: 4 SEP 2012
- Article first published online: 4 JUL 2012
- Accepted manuscript online: 12 JUN 2012 12:04AM EST
- Received 9 April, 2010; revised 23 May, 2012; accepted 31 May, 2012.
Fig. S1. Neighbour-joining trees for nearly full-length 16S rRNA gene sequences that were constructed based on minimum length nucleotide sequences. Bootstrap values (1000 replications) above 50% are given. The scale bars indicate 0.5–1% estimated sequence divergence.
A. Polynucleobacter necessarius cluster (1335 nucleotides).
B. GKS98 cluster (1264 nucleotides).
C. Rhodoferax sp. BAL47 cluster (1394 nucleotides).
D. IRD18C08 cluster (1308 nucleotides).
E. Luna-1 and Luna-2 clusters (1338 nucleotides).
Table S1. Morphometric and water chemistry parameters of sampling sites.
Table S2. Substrate metabolism profile using Biolog analysis of isolated strains.
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