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Fig. S1. A. Alignment group I ‘large’ Rok proteins. Residues are highlighted in red when conserved in at least seven of the eleven Rok proteins. Abbreviations (accession numbers given in brackets): Rok_L_pumSAF, B. pumilus SAFR-032 (YP_001486564.1); Rok_L_BpumAT, B. pumilus ATCC 7061 (ZP_03052836.1); Rok_L_licATC, B. licheniformis ATCC 14580 (YP_078814.1); Rok_L_amyloF, B. amyloliquefaciens FZB42 (YP_001420994.1); Rok_L_amyloD, B. amyloliquefaciens DSM 7 (YP_003920096.1); Rok_L_subW23, B. subtilis subsp. spizizenii W23 (YP_003865820.1); Rok_L_spizAT, B. subtilis subsp. spizizenii ATCC 6633 (ZP_06872236.1); Rok_L_SubBSn5, B. subtilis BSn5 (YP_004207469.1); Rok_L_spizTU, B. subtilis subsp. spizizenii TU-B-10 (YP_004877032.1); Rok_L_168, B. subtilis subsp. subtilis 168 (NP_389307.1); Rok_L_B. atroph, B. atropheus 1942 (YP_003972862).

B. Alignment ‘small’ Rok proteins. Residues are highlighted in red when conserved in at least six of the nine Rok proteins. Abbreviations (accession numbers given in brackets): Rok_S_subW23, B. subtilis subsp. spizizenii W23 (YP_003864911); Rok_S_subspi, B. subtilis subsp. spizizenii TU-B-10 (004876112.1); Rok_S_spizAT, B. subtilis subsp. spizizenii ATCC 6633 (ZP_06875354.1); Rok_S_bacill, Bacillus sp. BT1B_CT2 (ZP_08000071.1); Rok_S_Lich14, B. licheniformis ATCC 14580 (YP_079175.1); Rok_S_coag 36, B. coagulans 36D1 (YP_004861201.1); Rok_S_Coag 2, B. coagulans 2–6 (YP_004568723); Rok_S_LS20, B. subtilis natto IFO 3335 plasmid pLS20 (YP_004243533), Rok_S_pseudo, B. pseudomycoides DSM 12442 (ZP_04153718.1).

Table S1. Strains and plasmids used.

Table S2. Oligonucleotides used.

FilenameFormatSizeDescription
emi2819_sm_Tables.doc114KSupporting info item
emi2819_sm_FigS1AB.pdf94KSupporting info item

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