Present address: Institut für Medizinische Mikrobiologie, Immunologie & Parasitologie, Abteilung Pharmazeutische Mikrobiologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, D-53115 Bonn, Germany.
Thiosulfate dehydrogenase: a widespread unusual acidophilic c-type cytochrome
Article first published online: 11 JUL 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Thematic issue: Sulfur Metabolism
Volume 14, Issue 10, pages 2673–2688, October 2012
How to Cite
Denkmann, K., Grein, F., Zigann, R., Siemen, A., Bergmann, J., van Helmont, S., Nicolai, A., Pereira, I. A. C. and Dahl, C. (2012), Thiosulfate dehydrogenase: a widespread unusual acidophilic c-type cytochrome. Environmental Microbiology, 14: 2673–2688. doi: 10.1111/j.1462-2920.2012.02820.x
- Issue published online: 3 OCT 2012
- Article first published online: 11 JUL 2012
- Accepted manuscript online: 15 JUN 2012 03:27AM EST
- Received 15 February, 2012; revised 6 June, 2012; accepted 10 June, 2012.
Figure S1. Kinetic properties of recombinant Alc. vinosum thiosulfate dehydrogenase. Velocity curves versus thiosulfate concentration at two different fixed ferricyanide concentrations.
Figure S2. pH dependence of reduction of Thiomonas TsdB with thiosulfate catalysed by Alc. vinosum TsdA. Assays were performed at 30°C in the presence of 37.8 μg ml−1 (1.8 μM) TsdB, 8 mM thiosulfate and 100 mM ammonium acetate buffer at the desired pH value. The reaction was started by addition of 0.57 μg ml−1 (22.4 nM) AvTsdA and followed as increase of absorbance at the TsdB Soret peak (417 nm).
Table S1. Bacterial strains and plasmids used in this study.
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