Fig. S1. Annotation of ‘VirR boxes’ in ECA1856 promoter and entD promoter. (A) The location of the two ‘VirR box’ motifs identified upstream of ECA1856 are indicated. One box is indicated in green and the other in orange. The ECA1856 translational start site is indicated in blue. In (B), the two motifs of the ‘VirR box’ are indicated in orange and the putative Fur binding site is indicated with a grey dashed line. The entD translational start site is indicated in blue.

Fig. S2. VirR does not shift altered versions of the ‘VirR box’. An annotated virR promoter is shown at the top of (A) and promoter fragments A–E are indicated below. In (B), equal amount of labelled DNA (2 nM) of each promoter fragment was shifted either in the presence or absence of VirR. Protein from the same purification was used in each reaction in the quantities indicated above.

Table S1. virR v wild type and expI/virR v wild type microarray results.

Table S2. Principle component analysis.

Table S3. Genes identified as part of the VirR regulon by chromatin immunoprecipitation.

Table S4. Annotated promoters with ‘VirR boxes’.

Table S5. Additional VirR targets identified computationally.

Table S6. Oligonucleotides used in this study.

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