SEARCH

SEARCH BY CITATION

Fig. S1. Negative controls were performed to test for chemical conversion of NO2- to N2O. First, autoclaved artificial seawater amended with either 50 or 500 μM NO2- was incubated for 6 h and analysed for N2O production by gas chromatography. Second, autoclaved dissected shells were added to the autoclaved artificial seawater amended with either 50 or 500 μM NO2- and then analysed for N2O production. In none of the negative controls a significant increase in N2O could be detected during the incubation period of 6 h Thus, chemical conversion of NO2- to N2O can be ruled out as an explanation for the very high N2O production by live shell biofilms.

Table S1. Correlation coefficients between N2O production rate of dissected shells (nmol g−1 h−1), protein content of dissected shells (mg g−1) and DIN concentration in the water column of the microcosms (μM). n = 80 (4 microcosms × 5 sampling days × 4 replicate N2O measurements).

Table S2. t-test comparison of the N2O production rates in the reciprocal 4 h incubation of A+ and A− shells in seawater from the respective microcosm and in S− seawater, and of S+ and S− shells in seawater from the respective microcosm and in A+ seawater.

FilenameFormatSizeDescription
emi2823_sm_figs1-tabs1-2.doc57KSupporting info item
emi2823_sm_figs1.eps13425KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.