Shell biofilm-associated nitrous oxide production in marine molluscs: processes, precursors and relative importance
Article first published online: 26 JUL 2012
© 2012 John Wiley & Sons Ltd and Society for Applied Microbiology
Thematic Issue: Invertebrate-Microbe Interactions
Volume 15, Issue 7, pages 1943–1955, July 2013
How to Cite
Heisterkamp, I. M., Schramm, A., Larsen, L. H., Svenningsen, N. B., Lavik, G., de Beer, D. and Stief, P. (2013), Shell biofilm-associated nitrous oxide production in marine molluscs: processes, precursors and relative importance. Environmental Microbiology, 15: 1943–1955. doi: 10.1111/j.1462-2920.2012.02823.x
- Issue published online: 4 JUL 2013
- Article first published online: 26 JUL 2012
- Accepted manuscript online: 22 JUN 2012 10:53AM EST
- Received 29 December, 2011; revised 6 June, 2012; accepted 10 June, 2012.
Fig. S1. Negative controls were performed to test for chemical conversion of NO2- to N2O. First, autoclaved artificial seawater amended with either 50 or 500 μM NO2- was incubated for 6 h and analysed for N2O production by gas chromatography. Second, autoclaved dissected shells were added to the autoclaved artificial seawater amended with either 50 or 500 μM NO2- and then analysed for N2O production. In none of the negative controls a significant increase in N2O could be detected during the incubation period of 6 h Thus, chemical conversion of NO2- to N2O can be ruled out as an explanation for the very high N2O production by live shell biofilms.
Table S1. Correlation coefficients between N2O production rate of dissected shells (nmol g−1 h−1), protein content of dissected shells (mg g−1) and DIN concentration in the water column of the microcosms (μM). n = 80 (4 microcosms × 5 sampling days × 4 replicate N2O measurements).
Table S2. t-test comparison of the N2O production rates in the reciprocal 4 h incubation of A+ and A− shells in seawater from the respective microcosm and in S− seawater, and of S+ and S− shells in seawater from the respective microcosm and in A+ seawater.
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