Present address: UMR Inserm 1071, CBRV, 28 place Henri Dunant, 63001 Clermont-Ferrand, France. Disclosures: The authors have declared that no conflict of interest exists.
Bile salts induce long polar fimbriae expression favouring Crohn's disease-associated adherent-invasive Escherichia coli interaction with Peyer's patches
Article first published online: 13 JUL 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 355–371, February 2013
How to Cite
Chassaing, B., Etienne-Mesmin, L., Bonnet, R. and Darfeuille-Michaud, A. (2013), Bile salts induce long polar fimbriae expression favouring Crohn's disease-associated adherent-invasive Escherichia coli interaction with Peyer's patches. Environmental Microbiology, 15: 355–371. doi: 10.1111/j.1462-2920.2012.02824.x
- Issue published online: 28 JAN 2013
- Article first published online: 13 JUL 2012
- Accepted manuscript online: 22 JUN 2012 10:58AM EST
- Received 23 December, 2011; accepted 15 June, 2012.
Fig. S1. A. Activation of LF63 lpf operon promoter in wild-type bacteria and Δ-rscB isogenic mutant bacteria growth in EMEM culture medium containing 2% sodium choleate. Data are presented as the β-galactosidase activity at 6 h and 24 h, relative to the β-galactosidase activity at 2 h.
B. Activation of LF63 lpf operon promoter after LF63 + pBAD24 and LF63 + pBAD24-rcsB bacterial growth in EMEM culture medium containing 0.00%, 0.01%, 0.10% or 1.00% arabinose. Data are presented as the β-galactosidase activity at 6 h and 24 h, relative to the β-galactosidase activity at 2 h. Data are mean ± SEM of three separate experiments.
C. Competitive index of LF63 + pBAD24 and LF63 + pBAD24-rcsB. Intestinal ileal loops containing PP were inoculated by mixed inoculums comprising equivalent numbers of two bacterial strains, and their presence was compared after 5 h by competitive index analysis, which provides a sensitive measurement of the relative degree of attenuation.
Fig. S2. Transmission electron micrographs of negatively stained bacteria after growth of bacteria in cell culture medium with or without 2% sodium choleate. Bar: 500 nm.
Fig. S3. Motility assay of LF82 and LF82-ΔfhlA isogenic mutant on 0.3% agar after growth of bacteria in cell culture medium with or without 2% sodium choleate. Motility was visualized as a halo radial diffusion of bacteria around the primary inoculum.
Table S1. Putative FhlA binding sites in the genome of AIEC reference strain LF82.
Table S2. Bacterial strains and plasmids used in this study.
Table S3. Oligonucleotides used and PCR product sizes.
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