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Fig. S1. A. Activation of LF63 lpf operon promoter in wild-type bacteria and Δ-rscB isogenic mutant bacteria growth in EMEM culture medium containing 2% sodium choleate. Data are presented as the β-galactosidase activity at 6 h and 24 h, relative to the β-galactosidase activity at 2 h.

B. Activation of LF63 lpf operon promoter after LF63 + pBAD24 and LF63 + pBAD24-rcsB bacterial growth in EMEM culture medium containing 0.00%, 0.01%, 0.10% or 1.00% arabinose. Data are presented as the β-galactosidase activity at 6 h and 24 h, relative to the β-galactosidase activity at 2 h. Data are mean ± SEM of three separate experiments.

C. Competitive index of LF63 + pBAD24 and LF63 + pBAD24-rcsB. Intestinal ileal loops containing PP were inoculated by mixed inoculums comprising equivalent numbers of two bacterial strains, and their presence was compared after 5 h by competitive index analysis, which provides a sensitive measurement of the relative degree of attenuation.

Fig. S2. Transmission electron micrographs of negatively stained bacteria after growth of bacteria in cell culture medium with or without 2% sodium choleate. Bar: 500 nm.

Fig. S3. Motility assay of LF82 and LF82-ΔfhlA isogenic mutant on 0.3% agar after growth of bacteria in cell culture medium with or without 2% sodium choleate. Motility was visualized as a halo radial diffusion of bacteria around the primary inoculum.

Table S1. Putative FhlA binding sites in the genome of AIEC reference strain LF82.

Table S2. Bacterial strains and plasmids used in this study.

Table S3. Oligonucleotides used and PCR product sizes.

FilenameFormatSizeDescription
emi2824_sm_FS1.tif3315KSupporting info item
emi2824_sm_FS2.tif7317KSupporting info item
emi2824_sm_FS3.tif21487KSupporting info item
emi2824_sm_TS1.pdf6814KSupporting info item
emi2824_sm_TS2.doc77KSupporting info item
emi2824_sm_TS3.doc69KSupporting info item

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