Mollicutes-related endobacteria thrive inside liverwort-associated arbuscular mycorrhizal fungi
Version of Record online: 26 JUL 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Plant–Microbe Interactions
Volume 15, Issue 3, pages 822–836, March 2013
How to Cite
Desirò, A., Naumann, M., Epis, S., Novero, M., Bandi, C., Genre, A. and Bonfante, P. (2013), Mollicutes-related endobacteria thrive inside liverwort-associated arbuscular mycorrhizal fungi. Environmental Microbiology, 15: 822–836. doi: 10.1111/j.1462-2920.2012.02833.x
- Issue online: 4 MAR 2013
- Version of Record online: 26 JUL 2012
- Accepted manuscript online: 3 JUL 2012 10:38AM EST
- Received 18 April, 2012; revised 19 June, 2012; accepted 25 June, 2012.
Fig. S1. An arbuscular mycorrhizal fungus colonising L. cruciata (B, C) and C. conicum (A, D) as seen under transmission electron microscope. (A) The endophyte shows a coiled-like morphology, with hyphae having the same diameter. The fungus is always surrounded by a perifungal membrane (arrows); Liverwort cell lumen (cl). (B) Detail of hypha engaged in a cell to cell transition; perifungal membrane (arrows), liverwort cell lumen (cl). (C, D) Collapsed and degenerated hyphae (ch) and recolonization events of old hyphae (oh) by new ones (arrowheads) were observed. cl: liverwort cell lumen. Scale bars: (A) 2.7 μm; (B, D) 2.3 μm; (C) 3 μm.
Fig. S2. FISH labelling of AM fungi and BLOs inside a section of L. cruciata. Bright-field (A), red (B) and green (C) fluorescent images corresponding to Fig. 4A and B. BLOs are visible in (B) as red fluorescent coccoid spots (arrowheads), labelled by the BLO-specific probe BLOsADf2. The fungal cytoplasm (black arrowheads) is labelled in green (C) by the AMF-specific probe AML2ADf. cw: liverwort cell wall. Bars: 15 μm.
Fig. S3. FISH of BLOs inside a section of L. cruciata. (A) Bright-field image showing an arbuscule (ar) inside a parenchyma cell. (B) BLOsADf2 probe fluorescence (red) highlights red coccoid dots (arrowheads) in some of the arbuscule hyphae; a diffuse reddish autofluorescence marks the liverwort cell walls (cw). (C) The Buchnera-specific ApisP2a probe (green) was used as a negative control; no specific signal can be discriminated. (D) Overlay of the red and green fluorescence shown in (B) and (C). Bars: 15 μm.
|emi2833_sm_FigS1.tif||5911K||Supporting info item|
|emi2833_sm_FigS2.tif||1783K||Supporting info item|
|emi2833_sm_FigS3.tif||5384K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.