SEARCH

SEARCH BY CITATION

Fig. S1. An arbuscular mycorrhizal fungus colonising L. cruciata (B, C) and C. conicum (A, D) as seen under transmission electron microscope. (A) The endophyte shows a coiled-like morphology, with hyphae having the same diameter. The fungus is always surrounded by a perifungal membrane (arrows); Liverwort cell lumen (cl). (B) Detail of hypha engaged in a cell to cell transition; perifungal membrane (arrows), liverwort cell lumen (cl). (C, D) Collapsed and degenerated hyphae (ch) and recolonization events of old hyphae (oh) by new ones (arrowheads) were observed. cl: liverwort cell lumen. Scale bars: (A) 2.7 μm; (B, D) 2.3 μm; (C) 3 μm.

Fig. S2. FISH labelling of AM fungi and BLOs inside a section of L. cruciata. Bright-field (A), red (B) and green (C) fluorescent images corresponding to Fig. 4A and B. BLOs are visible in (B) as red fluorescent coccoid spots (arrowheads), labelled by the BLO-specific probe BLOsADf2. The fungal cytoplasm (black arrowheads) is labelled in green (C) by the AMF-specific probe AML2ADf. cw: liverwort cell wall. Bars: 15 μm.

Fig. S3. FISH of BLOs inside a section of L. cruciata. (A) Bright-field image showing an arbuscule (ar) inside a parenchyma cell. (B) BLOsADf2 probe fluorescence (red) highlights red coccoid dots (arrowheads) in some of the arbuscule hyphae; a diffuse reddish autofluorescence marks the liverwort cell walls (cw). (C) The Buchnera-specific ApisP2a probe (green) was used as a negative control; no specific signal can be discriminated. (D) Overlay of the red and green fluorescence shown in (B) and (C). Bars: 15 μm.

FilenameFormatSizeDescription
emi2833_sm_FigS1.tif5911KSupporting info item
emi2833_sm_FigS2.tif1783KSupporting info item
emi2833_sm_FigS3.tif5384KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.