Current address: Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Groundwater Ecology, Neuherberg, Germany.
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Research Article
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Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomics
Article first published online: 30 JUL 2012
DOI: 10.1111/j.1462-2920.2012.02836.x
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Issue
Environmental Microbiology
Special Issue: Marine Microbial Ecophysiology and Metagenomics
Volume 15, Issue 5, pages 1428–1440, May 2013
Additional Information
How to Cite
Hurwitz, B. L., Deng, L., Poulos, B. T. and Sullivan, M. B. (2013), Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomics. Environmental Microbiology, 15: 1428–1440. doi: 10.1111/j.1462-2920.2012.02836.x
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Current address: Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Groundwater Ecology, Neuherberg, Germany.
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Publication History
- Issue published online: 18 APR 2013
- Article first published online: 30 JUL 2012
- Accepted manuscript online: 9 JUL 2012 07:58AM EST
- Received 30 November, 2011; revised 29 June, 2012; accepted 30 June, 2012.
- Abstract
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Fig. S1. Superkingdom taxonomic profile across triplicate samples from each of the four viral metagenome methods and a non-replicated microbial metagenome. Note that these data represent only those metagenomic reads that had a significant hit to the SIMAP database.
Fig. S2. Fragment recruitment plots that show the distribution of metagenomic sequence reads that map to five abundant microbial genomes.
Table S1. The results of a one-way analysis of variance for the top taxonomic levels based on rank abundance in the samples by superkingdom, family and genus. Significant results are shown in bold. Abbreviations are used for each method: FC = FeCl3 CsCl + DNase, FS = FeCl3 sucrose + DNase, FD = FeCl3 DNase, TC = TFF and CsCl + DNase and TF = TFF CsCl + DNase.
| Filename | Format | Size | Description |
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| emi2836_sm_FigS1.eps | 834K | Supporting info item | |
| emi2836_sm_FigS2.eps | 8020K | Supporting info item | |
| emi2836_sm_TabS1.doc | 63K | Supporting info item |
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