Current address: Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Groundwater Ecology, Neuherberg, Germany.
Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomics
Version of Record online: 30 JUL 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Marine Microbial Ecophysiology and Metagenomics
Volume 15, Issue 5, pages 1428–1440, May 2013
How to Cite
Hurwitz, B. L., Deng, L., Poulos, B. T. and Sullivan, M. B. (2013), Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomics. Environmental Microbiology, 15: 1428–1440. doi: 10.1111/j.1462-2920.2012.02836.x
Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms
- Issue online: 18 APR 2013
- Version of Record online: 30 JUL 2012
- Accepted manuscript online: 9 JUL 2012 07:58AM EST
- Received 30 November, 2011; revised 29 June, 2012; accepted 30 June, 2012.
Fig. S1. Superkingdom taxonomic profile across triplicate samples from each of the four viral metagenome methods and a non-replicated microbial metagenome. Note that these data represent only those metagenomic reads that had a significant hit to the SIMAP database.
Fig. S2. Fragment recruitment plots that show the distribution of metagenomic sequence reads that map to five abundant microbial genomes.
Table S1. The results of a one-way analysis of variance for the top taxonomic levels based on rank abundance in the samples by superkingdom, family and genus. Significant results are shown in bold. Abbreviations are used for each method: FC = FeCl3 CsCl + DNase, FS = FeCl3 sucrose + DNase, FD = FeCl3 DNase, TC = TFF and CsCl + DNase and TF = TFF CsCl + DNase.
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